2hhc

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[[Image:2hhc.jpg|left|200px]]<br /><applet load="2hhc" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hhc, resolution 1.54&Aring;" />
 
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'''Crystal structure of fucosyltransferase NodZ from Bradyrhizobium'''<br />
 
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==Overview==
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==Crystal structure of fucosyltransferase NodZ from Bradyrhizobium==
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The fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes alpha1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain. Mutational data combined with structural and sequence alignments indicate residues of potential importance in GDP-fucose binding or in the catalytic mechanism. They are all clustered in three conserved sequence motifs located in the C-terminal domain.
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<StructureSection load='2hhc' size='340' side='right'caption='[[2hhc]], [[Resolution|resolution]] 1.54&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hhc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_sp._WM9 Bradyrhizobium sp. WM9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HHC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HHC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.54&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hhc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hhc OCA], [https://pdbe.org/2hhc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hhc RCSB], [https://www.ebi.ac.uk/pdbsum/2hhc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hhc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9AQ17_BRASW Q9AQ17_BRASW]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hh/2hhc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hhc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2HHC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bradyrhizobium_sp. Bradyrhizobium sp.] with <scene name='pdbligand=PO4:'>PO4</scene> and <scene name='pdbligand=TRS:'>TRS</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Glycoprotein_6-alpha-L-fucosyltransferase Glycoprotein 6-alpha-L-fucosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.68 2.4.1.68] Known structural/functional Sites: <scene name='pdbsite=AC1:Po4+Binding+Site+For+Residue+A+1001'>AC1</scene>, <scene name='pdbsite=AC2:Po4+Binding+Site+For+Residue+A+1002'>AC2</scene>, <scene name='pdbsite=AC3:Po4+Binding+Site+For+Residue+A+1003'>AC3</scene> and <scene name='pdbsite=AC4:Trs+Binding+Site+For+Residue+A+2001'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HHC OCA].
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*[[NodS|NodS]]
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__TOC__
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==Reference==
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</StructureSection>
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High-resolution structure of NodZ fucosyltransferase involved in the biosynthesis of the nodulation factor., Brzezinski K, Stepkowski T, Panjikar S, Bujacz G, Jaskolski M, Acta Biochim Pol. 2007;54(3):537-49. Epub 2007 Aug 30. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17762900 17762900]
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[[Category: Bradyrhizobium sp. WM9]]
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[[Category: Bradyrhizobium sp.]]
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[[Category: Large Structures]]
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[[Category: Glycoprotein 6-alpha-L-fucosyltransferase]]
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[[Category: Brzezinski K]]
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[[Category: Single protein]]
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[[Category: Bujacz G]]
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[[Category: Brzezinski, K.]]
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[[Category: Jaskolski M]]
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[[Category: Bujacz, G.]]
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[[Category: Panjikar S]]
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[[Category: Jaskolski, M.]]
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[[Category: Stepkowski T]]
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[[Category: Panjikar, S.]]
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[[Category: Stepkowski, T.]]
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[[Category: PO4]]
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[[Category: TRS]]
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[[Category: fucosyltransferase]]
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[[Category: glycosyltransferase]]
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[[Category: nodulation]]
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[[Category: nodz]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 27 07:48:07 2008''
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Current revision

Crystal structure of fucosyltransferase NodZ from Bradyrhizobium

PDB ID 2hhc

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