3b5w

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[[Image:3b5w.jpg|left|200px]]<br /><applet load="3b5w" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="3b5w, resolution 5.300&Aring;" />
 
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'''Crystal Structure of Eschericia coli MsbA'''<br />
 
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==Overview==
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==Crystal Structure of Eschericia coli MsbA==
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ATP-binding cassette (ABC) transporters are integral membrane proteins that translocate a wide variety of substrates across cellular membranes and are conserved from bacteria to humans. Here we compare four x-ray structures of the bacterial ABC lipid flippase, MsbA, trapped in different conformations, two nucleotide-bound structures and two in the absence of nucleotide. Comparison of the nucleotide-free conformations of MsbA reveals a flexible hinge formed by extracellular loops 2 and 3. This hinge allows the nucleotide-binding domains to disassociate while the ATP-binding half sites remain facing each other. The binding of the nucleotide causes a packing rearrangement of the transmembrane helices and changes the accessibility of the transporter from cytoplasmic (inward) facing to extracellular (outward) facing. The inward and outward openings are mediated by two different sets of transmembrane helix interactions. Altogether, the conformational changes between these structures suggest that large ranges of motion may be required for substrate transport.
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<StructureSection load='3b5w' size='340' side='right'caption='[[3b5w]], [[Resolution|resolution]] 5.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[3b5w]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B5W FirstGlance]. <br>
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3B5W is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B5W OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 5.3&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b5w OCA], [https://pdbe.org/3b5w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b5w RCSB], [https://www.ebi.ac.uk/pdbsum/3b5w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b5w ProSAT]</span></td></tr>
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==Reference==
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</table>
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Flexibility in the ABC transporter MsbA: Alternating access with a twist., Ward A, Reyes CL, Yu J, Roth CB, Chang G, Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):19005-10. Epub 2007 Nov 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18024585 18024585]
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== Function ==
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[https://www.uniprot.org/uniprot/MSBA_ECOLI MSBA_ECOLI] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b5/3b5w_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b5w ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Chang, G.]]
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[[Category: Chang G]]
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[[Category: Reyes, C L.]]
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[[Category: Reyes CL]]
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[[Category: Roth, C B.]]
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[[Category: Roth CB]]
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[[Category: Ward, A.]]
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[[Category: Ward A]]
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[[Category: Yu, J.]]
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[[Category: Yu J]]
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[[Category: abc transporter]]
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[[Category: atp-binding]]
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[[Category: hydrolase]]
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[[Category: inner membrane]]
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[[Category: lipid flippase]]
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[[Category: lipid transport]]
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[[Category: membrane]]
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[[Category: membrane protein]]
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[[Category: msba]]
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[[Category: nucleotide-binding]]
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[[Category: transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 27 07:48:28 2008''
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Current revision

Crystal Structure of Eschericia coli MsbA

PDB ID 3b5w

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