3wgt
From Proteopedia
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| - | {{STRUCTURE_3wgt| PDB=3wgt | SCENE= }} | ||
| - | ===Crystal structure of D-amino acid oxidase mutant=== | ||
| - | {{ABSTRACT_PUBMED_24644036}} | ||
| - | == | + | ==Crystal structure of D-amino acid oxidase mutant== |
| - | [[http://www.uniprot.org/uniprot/OXDA_PIG OXDA_PIG | + | <StructureSection load='3wgt' size='340' side='right'caption='[[3wgt]], [[Resolution|resolution]] 1.88Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[3wgt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WGT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WGT FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=QSC:(1R)-1-PHENYLETHANAMINE'>QSC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wgt OCA], [https://pdbe.org/3wgt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wgt RCSB], [https://www.ebi.ac.uk/pdbsum/3wgt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wgt ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/OXDA_PIG OXDA_PIG] Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The deracemization of racemic amines to yield enantioenriched amines using S-stereoselective amine oxidases (AOx) has recently been attracting attention. However, R-stereoselective AOx that are suitable for deracemization have not yet been identified. An R-stereoselective AOx was now evolved from porcine kidney D-amino acid oxidase (pkDAO) and subsequently use for the deracemization of racemic amines. The engineered pkDAO, which was obtained by directed evolution, displayed a markedly changed substrate specificity towards R amines. The mutant enzyme exhibited a high preference towards the substrate alpha-methylbenzylamine and was used to synthesize the S amine through deracemization. The findings of this study indicate that further investigations on the structure-activity relationship of AOx are warranted and also provide a new method for biotransformations in organic synthesis. | ||
| - | + | Tailoring D-Amino Acid Oxidase from the Pig Kidney to R-Stereoselective Amine Oxidase and its Use in the Deracemization of alpha-Methylbenzylamine.,Yasukawa K, Nakano S, Asano Y Angew Chem Int Ed Engl. 2014 Mar 18. doi: 10.1002/anie.201308812. PMID:24644036<ref>PMID:24644036</ref> | |
| - | + | ||
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | < | + | </div> |
| - | [[ | + | <div class="pdbe-citations 3wgt" style="background-color:#fffaf0;"></div> |
| - | + | ||
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Amino acid oxidase 3D structures|Amino acid oxidase 3D structures]] |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Sus scrofa]] | ||
| + | [[Category: Asano Y]] | ||
| + | [[Category: Nakano S]] | ||
| + | [[Category: Yasukawa K]] | ||
Current revision
Crystal structure of D-amino acid oxidase mutant
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