2rfc

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[[Image:2rfc.jpg|left|200px]]<br /><applet load="2rfc" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2rfc, resolution 3.10&Aring;" />
 
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'''Ligand bound (4-phenylimidazole) Crystal Structure of a Cytochrome P450 from the Thermoacidophilic Archaeon Picrophilus Torridus'''<br />
 
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==Overview==
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==Ligand bound (4-phenylimidazole) Crystal Structure of a Cytochrome P450 from the Thermoacidophilic Archaeon Picrophilus Torridus==
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The crystal structure of a cytochrome P450 from the thermoacidophile Picrophilus torridus, CYP231A2 (PTO1399), has been solved. This structure reveals a wide open substrate access channel. To better understand ligand-induced structural transitions in CYP231A2, protein-ligand interactions were investigated using 4-phenylimidazole. Comparison of the ligand-free and -bound CYP231A2 structures shows conformational changes where the F and G helices swing as a single rigid body about a pivot point at the N-terminal end of the F helix, allowing the F helix region to dip toward the heme, resulting in closer contacts with the ligand. Thermal melting data illustrate that the melting temperature for CYP231A2 increases nearly 10 degrees C upon ligand binding, thus illustrating that the closed conformation is substantially more stable. Furthermore, spectroscopic data indicate that the active site is stable at pH 4.5, although, unusually, the thiolate ligand to the iron can be reversibly protonated. CYP231A2 does not exhibit structural features normally associated with thermophilic proteins such as an increase in salt bridge networks or extensive aromatic clustering. The increase in thermal stability instead is best correlated with the smaller size and shorter loops in CYP231A2 compared to other P450s.
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<StructureSection load='2rfc' size='340' side='right'caption='[[2rfc]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2rfc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Picrophilus_torridus Picrophilus torridus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RFC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=PIM:4-PHENYL-1H-IMIDAZOLE'>PIM</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rfc OCA], [https://pdbe.org/2rfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rfc RCSB], [https://www.ebi.ac.uk/pdbsum/2rfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rfc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q6KZ68_PICTO Q6KZ68_PICTO]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rf/2rfc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rfc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2RFC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Picrophilus_torridus Picrophilus torridus] with <scene name='pdbligand=HEM:'>HEM</scene> and <scene name='pdbligand=PIM:'>PIM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Unspecific_monooxygenase Unspecific monooxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.14.1 1.14.14.1] Known structural/functional Sites: <scene name='pdbsite=AC1:Hem+Binding+Site+For+Residue+A+410'>AC1</scene>, <scene name='pdbsite=AC2:Pim+Binding+Site+For+Residue+A+411'>AC2</scene>, <scene name='pdbsite=AC3:Hem+Binding+Site+For+Residue+B+410'>AC3</scene>, <scene name='pdbsite=AC4:Pim+Binding+Site+For+Residue+B+411'>AC4</scene>, <scene name='pdbsite=AC5:Hem+Binding+Site+For+Residue+C+410'>AC5</scene>, <scene name='pdbsite=AC6:Pim+Binding+Site+For+Residue+C+411'>AC6</scene>, <scene name='pdbsite=AC7:Hem+Binding+Site+For+Residue+D+410'>AC7</scene> and <scene name='pdbsite=AC8:Pim+Binding+Site+For+Residue+D+411'>AC8</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RFC OCA].
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*[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal Structure and Properties of CYP231A2 from the Thermoacidophilic Archaeon Picrophilus torridus(,)., Ho WW, Li H, Nishida CR, Montellano PR, Poulos TL, Biochemistry. 2008 Feb 19;47(7):2071-9. Epub 2008 Jan 16. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18197710 18197710]
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[[Category: Large Structures]]
[[Category: Picrophilus torridus]]
[[Category: Picrophilus torridus]]
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[[Category: Single protein]]
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[[Category: Ho WW]]
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[[Category: Unspecific monooxygenase]]
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[[Category: Li H]]
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[[Category: Ho, W W.]]
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[[Category: Nishida CR]]
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[[Category: Li, H.]]
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[[Category: Ortiz de Montellano PR]]
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[[Category: Montellano, P R.Ortiz de.]]
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[[Category: Poulos TL]]
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[[Category: Nishida, C R.]]
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[[Category: Poulos, T L.]]
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[[Category: HEM]]
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[[Category: PIM]]
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[[Category: cytochrome p450 thermophile]]
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[[Category: heme]]
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[[Category: iron]]
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[[Category: metal-binding]]
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[[Category: monooxygenase]]
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[[Category: oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 27 07:48:43 2008''
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Current revision

Ligand bound (4-phenylimidazole) Crystal Structure of a Cytochrome P450 from the Thermoacidophilic Archaeon Picrophilus Torridus

PDB ID 2rfc

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