2rdt

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(New page: 200px<br /><applet load="2rdt" size="350" color="white" frame="true" align="right" spinBox="true" caption="2rdt, resolution 1.95&Aring;" /> '''Crystal Structure of...)
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[[Image:2rdt.jpg|left|200px]]<br /><applet load="2rdt" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2rdt, resolution 1.95&Aring;" />
 
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'''Crystal Structure of Human Glycolate Oxidase (GO) in Complex with CDST'''<br />
 
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==Overview==
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==Crystal Structure of Human Glycolate Oxidase (GO) in Complex with CDST==
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Human glycolate oxidase (GO) catalyzes the FMN-dependent oxidation of glycolate to glyoxylate and glyoxylate to oxalate, a key metabolite in kidney stone formation. We report herein the structures of recombinant GO complexed with sulfate, glyoxylate, and an inhibitor, 4-carboxy-5-dodecylsulfanyl-1,2,3-triazole (CDST), determined by X-ray crystallography. In contrast to most alpha-hydroxy acid oxidases including spinach glycolate oxidase, a loop region, known as loop 4, is completely visible when the GO active site contains a small ligand. The lack of electron density for this loop in the GO-CDST complex, which mimics a large substrate, suggests that a disordered to ordered transition may occur with the binding of substrates. The conformational flexibility of Trp110 appears to be responsible for enabling GO to react with alpha-hydroxy acids of various chain lengths. Moreover, the movement of Trp110 disrupts a hydrogen-bonding network between Trp110, Leu191, Tyr134, and Tyr208. This loss of interactions is the first indication that active site movements are directly linked to changes in the conformation of loop 4. The kinetic parameters for the oxidation of glycolate, glyoxylate, and 2-hydroxy octanoate indicate that the oxidation of glycolate to glyoxylate is the primary reaction catalyzed by GO, while the oxidation of glyoxylate to oxalate is most likely not relevant under normal conditions. However, drugs that exploit the unique structural features of GO may ultimately prove to be useful for decreasing glycolate and glyoxylate levels in primary hyperoxaluria type 1 patients who have the inability to convert peroxisomal glyoxylate to glycine.
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<StructureSection load='2rdt' size='340' side='right'caption='[[2rdt]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2rdt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RDT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2RD:5-(DODECYLTHIO)-1H-1,2,3-TRIAZOLE-4-CARBOXYLIC+ACID'>2RD</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rdt OCA], [https://pdbe.org/2rdt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rdt RCSB], [https://www.ebi.ac.uk/pdbsum/2rdt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rdt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/HAOX1_HUMAN HAOX1_HUMAN] Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rd/2rdt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rdt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2RDT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FMN:'>FMN</scene> and <scene name='pdbligand=2RD:'>2RD</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/(S)-2-hydroxy-acid_oxidase (S)-2-hydroxy-acid oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.3.15 1.1.3.15] Known structural/functional Sites: <scene name='pdbsite=AC1:Fmn+Binding+Site+For+Residue+A+364'>AC1</scene> and <scene name='pdbsite=AC2:2rd+Binding+Site+For+Residue+A+365'>AC2</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RDT OCA].
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*[[Glycolate oxidase 3D structures|Glycolate oxidase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Active Site and Loop 4 Movements within Human Glycolate Oxidase: Implications for Substrate Specificity and Drug Design., Murray MS, Holmes RP, Lowther WT, Biochemistry. 2008 Feb 26;47(8):2439-49. Epub 2008 Jan 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18215067 18215067]
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[[Category: (S)-2-hydroxy-acid oxidase]]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Holmes, R P.]]
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[[Category: Holmes RP]]
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[[Category: Lowther, W T.]]
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[[Category: Lowther WT]]
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[[Category: Murray, M S.]]
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[[Category: Murray MS]]
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[[Category: 2RD]]
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[[Category: FMN]]
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[[Category: flavoprotein]]
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[[Category: fmn]]
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[[Category: glycolate pathway]]
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[[Category: oxidoreductase]]
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[[Category: peroxisome]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 27 07:59:07 2008''
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Current revision

Crystal Structure of Human Glycolate Oxidase (GO) in Complex with CDST

PDB ID 2rdt

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