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4csg
From Proteopedia
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| - | {{STRUCTURE_4csg| PDB=4csg | SCENE= }} | ||
| - | ===Structural insights into Toscana virus RNA encapsidation=== | ||
| - | {{ABSTRACT_PUBMED_24688060}} | ||
| - | == | + | ==Structural insights into Toscana virus RNA encapsidation== |
| - | [[4csg]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CSG OCA]. | + | <StructureSection load='4csg' size='340' side='right'caption='[[4csg]], [[Resolution|resolution]] 3.32Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4csg]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Toscana_virus Toscana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CSG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CSG FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.32Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4csg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4csg OCA], [https://pdbe.org/4csg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4csg RCSB], [https://www.ebi.ac.uk/pdbsum/4csg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4csg ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/NCAP_TOSV NCAP_TOSV] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Toscana virus is an emerging bunyavirus in Mediterranean Europe where it accounts for 80% of pediatric meningitis cases during the summer. The negative-strand ribonucleic acid (RNA) genome of the virus is wrapped around the virally encoded nucleoprotein N to form the ribonucleoprotein complex (RNP). We determined crystal structures of hexameric N alone (apo) and in complex with a nonameric single-stranded RNA. RNA is sequestered in a sequence-independent fashion in a deep groove inside the hexamer. At the junction between two adjacent copies of Ns, RNA binding induced an inter-subunit rotation, which opened the RNA-binding tunnel and created a new assembly interface at the outside of the hexamer. Based on these findings, we suggest a structural model for how binding of RNA to N promotes the formation of helical RNPs, which are a characteristic hallmark of many negative-strand RNA viruses. | ||
| - | + | Structural insights into RNA encapsidation and helical assembly of the Toscana virus nucleoprotein.,Olal D, Dick A, Woods VL Jr, Liu T, Li S, Devignot S, Weber F, Saphire EO, Daumke O Nucleic Acids Res. 2014 Mar 31. PMID:24688060<ref>PMID:24688060</ref> | |
| - | <ref | + | |
| - | [[Category: | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 4csg" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | |
| + | ==See Also== | ||
| + | *[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Toscana virus]] | ||
| + | [[Category: Daumke O]] | ||
| + | [[Category: Olal D]] | ||
Current revision
Structural insights into Toscana virus RNA encapsidation
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