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4jn9

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{{STRUCTURE_4jn9| PDB=4jn9 | SCENE= }}
 
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===Crystal structure of the DepH===
 
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{{ABSTRACT_PUBMED_24553401}}
 
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==About this Structure==
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==Crystal structure of the DepH==
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[[4jn9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JN9 OCA].
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<StructureSection load='4jn9' size='340' side='right'caption='[[4jn9]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4jn9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chromobacterium_violaceum Chromobacterium violaceum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4JN9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4JN9 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4jn9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4jn9 OCA], [https://pdbe.org/4jn9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4jn9 RCSB], [https://www.ebi.ac.uk/pdbsum/4jn9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4jn9 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/A4ZPY8_CHRVL A4ZPY8_CHRVL]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The disulfide bond is unusual in natural products and critical for thermal stability, cell permeability and bioactivity. DepH from Chromobacterium violaceum No. 968 is an FAD-dependent enzyme responsible for catalyzing the disulfide bond formation of FK228, an anticancer prodrug approved for the treatment of cutaneous T-cell lymphoma. Here we report the crystal structures of DepH and DepH complexed with a substrate analogue S,S'-dimethyl FK228 at 1.82 A and 2.00 A, respectively. Structural and biochemical analyses revealed that DepH, in contrast to the well characterized low molecular weight thioredoxin reductases (LMW TrxRs), is an NADP(+)-independent dithiol oxidase. DepH not only lacks a conserved GGGDXAXE motif necessary for NADP(+) binding in the canonical LMW TrxRs, but also contains a 11-residue sequence which physically impedes the binding of NADP(+). These observations explain the difference between NADP(+)-independent small molecule dithiol oxidases and NADP(+)-dependent thioredoxin reductases and provide insights for understanding the catalytic mechanism of dithiol oxidases involved in natural product biosynthesis.
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==Reference==
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The structural basis of an NADP(+)-independent dithiol oxidase in FK228 biosynthesis.,Li J, Wang C, Zhang ZM, Cheng YQ, Zhou J Sci Rep. 2014 Feb 20;4:4145. doi: 10.1038/srep04145. PMID:24553401<ref>PMID:24553401</ref>
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<ref group="xtra">PMID:024553401</ref><references group="xtra"/><references/>
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[[Category: Cheng, E.]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Li,J.]]
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</div>
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[[Category: Wang, C.]]
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<div class="pdbe-citations 4jn9" style="background-color:#fffaf0;"></div>
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[[Category: Zhang, Z M.]]
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== References ==
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[[Category: Zhou, J H.]]
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<references/>
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[[Category: Depsipeptide]]
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__TOC__
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[[Category: Disulfide bond formation]]
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</StructureSection>
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[[Category: Fad-dependent oxidoreductase]]
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[[Category: Chromobacterium violaceum]]
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[[Category: Fk228]]
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[[Category: Large Structures]]
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[[Category: Oxidoreductase]]
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[[Category: Cheng E]]
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[[Category: Li J]]
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[[Category: Wang C]]
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[[Category: Zhang ZM]]
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[[Category: Zhou JH]]

Current revision

Crystal structure of the DepH

PDB ID 4jn9

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