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4mqx

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{{STRUCTURE_4mqx| PDB=4mqx | SCENE= }}
 
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===CLC-ec1 Fab Complex Cysless A399C-A432C mutant===
 
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==Function==
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==CLC-ec1 Fab Complex Cysless A399C-A432C mutant==
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[[http://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI]] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>
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<StructureSection load='4mqx' size='340' side='right'caption='[[4mqx]], [[Resolution|resolution]] 3.52&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4mqx]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MQX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MQX FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mqx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mqx OCA], [https://pdbe.org/4mqx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mqx RCSB], [https://www.ebi.ac.uk/pdbsum/4mqx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mqx ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CLCA_ECOLI CLCA_ECOLI] Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme acid resistance (XAR) response.<ref>PMID:12384697</ref> <ref>PMID:14985752</ref> <ref>PMID:16341087</ref> <ref>PMID:16905147</ref> <ref>PMID:18678918</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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CLC-type exchangers mediate transmembrane Cl(-) transport. Mutations altering their gating properties cause numerous genetic disorders. However, their transport mechanism remains poorly understood. In conventional models, two gates alternatively expose substrates to the intra- or extracellular solutions. A glutamate was identified as the only gate in the CLCs, suggesting that CLCs function by a nonconventional mechanism. Here we show that transport in CLC-ec1, a prokaryotic homolog, is inhibited by cross-links constraining movement of helix O far from the transport pathway. Cross-linked CLC-ec1 adopts a wild-type-like structure, indicating stabilization of a native conformation. Movements of helix O are transduced to the ion pathway via a direct contact between its C terminus and a tyrosine that is a constitutive element of the second gate of CLC transporters. Therefore, the CLC exchangers have two gates that are coupled through conformational rearrangements outside the ion pathway.
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==About this Structure==
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Conformational changes required for H(+)/Cl(-) exchange mediated by a CLC transporter.,Basilio D, Noack K, Picollo A, Accardi A Nat Struct Mol Biol. 2014 May;21(5):456-63. doi: 10.1038/nsmb.2814. Epub 2014 Apr, 20. PMID:24747941<ref>PMID:24747941</ref>
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[[4mqx]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MQX OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<references group="xtra"/><references/>
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</div>
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[[Category: Accardi, A.]]
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<div class="pdbe-citations 4mqx" style="background-color:#fffaf0;"></div>
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[[Category: Basilio, D.]]
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== References ==
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[[Category: Noack, K.]]
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<references/>
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[[Category: Picollo, A.]]
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__TOC__
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[[Category: Alpha helical transmembrane protein]]
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</StructureSection>
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[[Category: Cl/h antiporter]]
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[[Category: Escherichia coli K-12]]
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[[Category: Membrane protein]]
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[[Category: Large Structures]]
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[[Category: Metal transport]]
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[[Category: Mus musculus]]
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[[Category: Accardi A]]
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[[Category: Basilio D]]
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[[Category: Noack K]]
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[[Category: Picollo A]]

Current revision

CLC-ec1 Fab Complex Cysless A399C-A432C mutant

PDB ID 4mqx

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