2hf7

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(New page: 200px<br /><applet load="2hf7" size="350" color="white" frame="true" align="right" spinBox="true" caption="2hf7, resolution 1.60&Aring;" /> '''Transition State Ana...)
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[[Image:2hf7.jpg|left|200px]]<br /><applet load="2hf7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hf7, resolution 1.60&Aring;" />
 
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'''Transition State Analogue of AphA class B Acid Phosphatase/Phosphotransferase (Aluminium Fluoride Complex)'''<br />
 
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==About this Structure==
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==Transition State Analogue of AphA class B Acid Phosphatase/Phosphotransferase (Aluminium Fluoride Complex)==
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2HF7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=AF3:'>AF3</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] Known structural/functional Sites: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Residue+A+700'>AC1</scene>, <scene name='pdbsite=AC2:Mg+Binding+Site+For+Residue+B+701'>AC2</scene>, <scene name='pdbsite=AC3:Af3+Binding+Site+For+Residue+A+800'>AC3</scene> and <scene name='pdbsite=AC4:Af3+Binding+Site+For+Residue+B+801'>AC4</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HF7 OCA].
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<StructureSection load='2hf7' size='340' side='right'caption='[[2hf7]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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[[Category: Acid phosphatase]]
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== Structural highlights ==
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[[Category: Escherichia coli]]
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<table><tr><td colspan='2'>[[2hf7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HF7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HF7 FirstGlance]. <br>
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[[Category: Single protein]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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[[Category: Benvenuti, M.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AF3:ALUMINUM+FLUORIDE'>AF3</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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[[Category: Calderone, V.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hf7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hf7 OCA], [https://pdbe.org/2hf7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hf7 RCSB], [https://www.ebi.ac.uk/pdbsum/2hf7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hf7 ProSAT]</span></td></tr>
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[[Category: Cappelletti, E.]]
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</table>
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[[Category: Leone, R.]]
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== Function ==
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[[Category: Mangani, S.]]
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[https://www.uniprot.org/uniprot/APHA_ECOLI APHA_ECOLI] Dephosphorylates several organic phosphate monoesters including 3'- and 5'-nucleotides, 2'-deoxy-5'-nucleotides, pNPP, phenyl phosphate, glycerol 2-phosphate, ribose 5-phosphate, O-phospho-L-amino acids and phytic acid, showing the highest activity with aryl phosphoesters (pNPP, phenyl phosphate and O-phospho-L-tyrosine), and to a lesser extent with 3'- and 5'-nucleotides. No activity toward ATP, phosphodiesters, glycerol-1-phosphate, glucose 1-phosphate, glucose 6-phosphate, NADP, GTP or 3',5'-cAMP, ADP or ATP. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds. Capable of transferring phosphate from either pNPP or UMP to adenosine or uridine. Does not exhibit nucleotide phosphomutase activity.<ref>PMID:9011040</ref> <ref>PMID:16297670</ref>
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[[Category: AF3]]
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== Evolutionary Conservation ==
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[[Category: MG]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: apha class b acid phosphatase/phosphotransferase - transition state analogue]]
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Check<jmol>
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[[Category: hydrolase]]
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hf/2hf7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hf7 ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Mar 5 13:20:28 2008''
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==See Also==
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*[[Acid phosphatase 3D structures|Acid phosphatase 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Benvenuti M]]
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[[Category: Calderone V]]
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[[Category: Cappelletti E]]
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[[Category: Leone R]]
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[[Category: Mangani S]]

Current revision

Transition State Analogue of AphA class B Acid Phosphatase/Phosphotransferase (Aluminium Fluoride Complex)

PDB ID 2hf7

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