1pru

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{{STRUCTURE_1pru| PDB=1pru | SCENE= }}
 
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===PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING===
 
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{{ABSTRACT_PUBMED_8591032}}
 
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==Function==
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==PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING==
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[[http://www.uniprot.org/uniprot/PURR_ECOLI PURR_ECOLI]] Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. In addition, it participates in the regulation or coregulation of genes involved in de novo pyrimidine nucleotide biosynthesis, salvage and uptake (pyrC, pyrD, carAB and codBA), and of several genes encoding enzymes necessary for nucleotide and polyamine biosynthesis (prsA, glyA, gcvTHP, speA, glnB). Binds to a 16-bp palindromic sequence located within the promoter region of pur regulon genes. The consensus binding sequence is 5'-ACGCAAACGTTTTCNT-3'. PurR is allosterically activated to bind its cognate DNA by binding the purine corepressors, hypoxanthine or guanine, thereby effecting transcription repression.<ref>PMID:2404765</ref> <ref>PMID:2211500</ref> <ref>PMID:1400170</ref> <ref>PMID:14741201</ref>
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<StructureSection load='1pru' size='340' side='right'caption='[[1pru]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1pru]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PRU FirstGlance]. <br>
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[[1pru]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PRU OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1pru FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pru OCA], [https://pdbe.org/1pru PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1pru RCSB], [https://www.ebi.ac.uk/pdbsum/1pru PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1pru ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PURR_ECOLI PURR_ECOLI] Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. In addition, it participates in the regulation or coregulation of genes involved in de novo pyrimidine nucleotide biosynthesis, salvage and uptake (pyrC, pyrD, carAB and codBA), and of several genes encoding enzymes necessary for nucleotide and polyamine biosynthesis (prsA, glyA, gcvTHP, speA, glnB). Binds to a 16-bp palindromic sequence located within the promoter region of pur regulon genes. The consensus binding sequence is 5'-ACGCAAACGTTTTCNT-3'. PurR is allosterically activated to bind its cognate DNA by binding the purine corepressors, hypoxanthine or guanine, thereby effecting transcription repression.<ref>PMID:2404765</ref> <ref>PMID:2211500</ref> <ref>PMID:1400170</ref> <ref>PMID:14741201</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/1pru_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1pru ConSurf].
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<div style="clear:both"></div>
==See Also==
==See Also==
*[[Purine repressor|Purine repressor]]
*[[Purine repressor|Purine repressor]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:008591032</ref><references group="xtra"/><references/>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Brennan, R G.]]
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[[Category: Large Structures]]
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[[Category: Enari, M.]]
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[[Category: Brennan RG]]
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[[Category: Kobayashi, K.]]
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[[Category: Enari M]]
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[[Category: Mizobuchi, K.]]
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[[Category: Kobayashi K]]
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[[Category: Morikawa, S.]]
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[[Category: Mizobuchi K]]
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[[Category: Nagadoi, A.]]
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[[Category: Morikawa S]]
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[[Category: Nakamura, H.]]
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[[Category: Nagadoi A]]
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[[Category: Nishimura, Y.]]
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[[Category: Nakamura H]]
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[[Category: Sampei, G.]]
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[[Category: Nishimura Y]]
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[[Category: Schumacher, M A.]]
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[[Category: Sampei G]]
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[[Category: Yamamoto, H.]]
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[[Category: Schumacher MA]]
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[[Category: Dna-binding protein]]
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[[Category: Yamamoto H]]
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[[Category: Purine repressor]]
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Current revision

PURINE REPRESSOR DNA-BINDING DOMAIN DNA BINDING

PDB ID 1pru

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