1n8j

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{{STRUCTURE_1n8j| PDB=1n8j | SCENE= }}
 
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===Crystal Structure of AhpC with Active Site Cysteine mutated to Serine (C46S)===
 
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{{ABSTRACT_PUBMED_12714747}}
 
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==Function==
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==Crystal Structure of AhpC with Active Site Cysteine mutated to Serine (C46S)==
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[[http://www.uniprot.org/uniprot/AHPC_SALTY AHPC_SALTY]] Directly reduces alkyl hydroperoxides with the use of electrons donated by the 57 kDa flavoprotein alkyl hydroperoxide reductase.
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<StructureSection load='1n8j' size='340' side='right'caption='[[1n8j]], [[Resolution|resolution]] 2.17&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1n8j]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N8J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.17&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n8j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n8j OCA], [https://pdbe.org/1n8j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n8j RCSB], [https://www.ebi.ac.uk/pdbsum/1n8j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n8j ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AHPC_SALTY AHPC_SALTY] Directly reduces alkyl hydroperoxides with the use of electrons donated by the 57 kDa flavoprotein alkyl hydroperoxide reductase.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n8/1n8j_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1n8j ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Eukaryotic 2-Cys peroxiredoxins (2-Cys Prxs) not only act as antioxidants, but also appear to regulate hydrogen peroxide-mediated signal transduction. We show that bacterial 2-Cys Prxs are much less sensitive to oxidative inactivation than are eukaryotic 2-Cys Prxs. By identifying two sequence motifs unique to the sensitive 2-Cys Prxs and comparing the crystal structure of a bacterial 2-Cys Prx at 2.2 angstrom resolution with other Prx structures, we define the structural origins of sensitivity. We suggest this adaptation allows 2-Cys Prxs to act as floodgates, keeping resting levels of hydrogen peroxide low, while permitting higher levels during signal transduction.
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==About this Structure==
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Peroxiredoxin evolution and the regulation of hydrogen peroxide signaling.,Wood ZA, Poole LB, Karplus PA Science. 2003 Apr 25;300(5619):650-3. PMID:12714747<ref>PMID:12714747</ref>
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[[1n8j]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_typhimurium Salmonella enterica subsp. enterica serovar typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N8J OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:012714747</ref><references group="xtra"/><references/>
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</div>
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[[Category: Peroxiredoxin]]
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<div class="pdbe-citations 1n8j" style="background-color:#fffaf0;"></div>
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[[Category: Salmonella enterica subsp. enterica serovar typhimurium]]
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== References ==
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[[Category: Karplus, P A.]]
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<references/>
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[[Category: Poole, L B.]]
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__TOC__
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[[Category: Wood, Z A.]]
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</StructureSection>
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[[Category: Ahpc]]
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[[Category: Large Structures]]
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[[Category: Ahpf]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
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[[Category: Alkylhydroperoxide reductase]]
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[[Category: Karplus PA]]
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[[Category: Antioxidant]]
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[[Category: Poole LB]]
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[[Category: Decamer]]
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[[Category: Wood ZA]]
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[[Category: Oxidoreductase]]
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[[Category: Peroxidase]]
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[[Category: Peroxiredoxin]]
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Current revision

Crystal Structure of AhpC with Active Site Cysteine mutated to Serine (C46S)

PDB ID 1n8j

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