1p33
From Proteopedia
(Difference between revisions)
| (5 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | {{STRUCTURE_1p33| PDB=1p33 | SCENE= }} | ||
| - | ===Pteridine reductase from Leishmania tarentolae complex with NADPH and MTX=== | ||
| - | {{ABSTRACT_PUBMED_12925782}} | ||
| - | == | + | ==Pteridine reductase from Leishmania tarentolae complex with NADPH and MTX== |
| - | [[http://www.uniprot.org/uniprot/PTR1_LEITA PTR1_LEITA | + | <StructureSection load='1p33' size='340' side='right'caption='[[1p33]], [[Resolution|resolution]] 2.86Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[1p33]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_tarentolae Leishmania tarentolae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1P33 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1P33 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.86Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MTX:METHOTREXATE'>MTX</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1p33 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p33 OCA], [https://pdbe.org/1p33 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1p33 RCSB], [https://www.ebi.ac.uk/pdbsum/1p33 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1p33 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PTR1_LEITA PTR1_LEITA] Exhibits a NADPH-dependent biopterin reductase activity. Has good activity with folate and significant activity with dihydrofolate and dihydrobiopterin, but not with quinonoid dihydrobiopterin. Confers resistance to methotrexate (MTX). | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p3/1p33_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1p33 ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The protozoan parasites Leishmania utilize a pteridine-reducing enzyme, pteridine reductase (PTR1), to bypass antifolate inhibition. The crystal structure of PTR1 from L. tarentolae has been solved as a binary complex with NADPH at 2.8 A resolution. The structure was solved by molecular-replacement techniques using the recently reported L. major PTR1 structure as a search model. Comparisons of the present structure with the L. major PTR1 allowed us to identify regions of flexibility in the molecule. PTR1 is a member of the growing family of short-chain dehydrogenases (SDR) which share the characteristic Tyr(Xaa)(3)Lys motif in the vicinity of the active site. The functional enzyme is a tetramer and the crystallographic asymmetric unit contains a tetramer with 222 point-group symmetry. | ||
| - | + | Structure of pteridine reductase (PTR1) from Leishmania tarentolae.,Zhao H, Bray T, Ouellette M, Zhao M, Ferre RA, Matthews D, Whiteley JM, Varughese KI Acta Crystallogr D Biol Crystallogr. 2003 Sep;59(Pt 9):1539-44. Epub 2003, Aug 19. PMID:12925782<ref>PMID:12925782</ref> | |
| - | + | ||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 1p33" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[Pteridine reductase|Pteridine reductase]] | *[[Pteridine reductase|Pteridine reductase]] | ||
| - | + | == References == | |
| - | == | + | <references/> |
| - | + | __TOC__ | |
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Leishmania tarentolae]] | [[Category: Leishmania tarentolae]] | ||
| - | + | [[Category: Bray T]] | |
| - | [[Category: Bray | + | [[Category: Ferre RA]] |
| - | [[Category: Ferre | + | [[Category: Matthews D]] |
| - | [[Category: Matthews | + | [[Category: Ouellette M]] |
| - | [[Category: Ouellette | + | [[Category: Varughese KI]] |
| - | [[Category: Varughese | + | [[Category: Whiteley JM]] |
| - | [[Category: Whiteley | + | [[Category: Zhao H]] |
| - | [[Category: Zhao | + | [[Category: Zhao M]] |
| - | [[Category: Zhao | + | |
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
| - | + | ||
Current revision
Pteridine reductase from Leishmania tarentolae complex with NADPH and MTX
| |||||||||||
