4erd
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| - | {{STRUCTURE_4erd| PDB=4erd | SCENE= }} | ||
| - | ===Crystal structure of the C-terminal domain of Tetrahymena telomerase protein p65 in complex with stem IV of telomerase RNA=== | ||
| - | {{ABSTRACT_PUBMED_22705372}} | ||
| - | == | + | ==Crystal structure of the C-terminal domain of Tetrahymena telomerase protein p65 in complex with stem IV of telomerase RNA== |
| - | [[4erd]] is a 4 chain structure with sequence from [ | + | <StructureSection load='4erd' size='340' side='right'caption='[[4erd]], [[Resolution|resolution]] 2.59Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4erd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Tetrahymena_thermophila Tetrahymena thermophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ERD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ERD FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.589Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4erd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4erd OCA], [https://pdbe.org/4erd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4erd RCSB], [https://www.ebi.ac.uk/pdbsum/4erd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4erd ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LARP7_TETTS LARP7_TETTS] RNA-binding protein required for assembly of the holoenzyme telomerase ribonucleoprotein (RNP) complex (PubMed:15131081, PubMed:15696174, PubMed:16507983, PubMed:17322903, PubMed:20713447, PubMed:22315458, PubMed:22705372). Telomerase is an essential ribonucleoprotein enzyme that copies new telomeric repeats onto chromosome ends by repetitively synthesizing the short telomere-repeat sequence 5'-TTGGGG-3' using an RNA template component TER (PubMed:15696174, PubMed:17322903, PubMed:20713447). TAP65/p65 specifically binds telomerase RNA template TER and is required for biogenesis and placement of the TER stem-terminus element: TAP65/p65 first protects the 3'-end of TER from degradation and acts as a chaperone to correctly fold TER for protein binding; it then bends TER stem-loop IV to position it for interaction of stem-loop IV with catalytic TERT RNA-binding domain (PubMed:15696174, PubMed:17322903, PubMed:20713447, PubMed:22315458, PubMed:22705372).<ref>PMID:15131081</ref> <ref>PMID:15696174</ref> <ref>PMID:16507983</ref> <ref>PMID:17322903</ref> <ref>PMID:20713447</ref> <ref>PMID:22315458</ref> <ref>PMID:22705372</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Telomerase is a ribonucleoprotein complex essential for maintenance of telomere DNA at linear chromosome ends. The catalytic core of Tetrahymena telomerase comprises a ternary complex of telomerase RNA (TER), telomerase reverse transcriptase (TERT), and the essential La family protein p65. NMR and crystal structures of p65 C-terminal domain and its complex with stem IV of TER reveal that RNA recognition is achieved by a combination of single- and double-stranded RNA binding, which induces a 105 degrees bend in TER. The domain is a cryptic, atypical RNA recognition motif with a disordered C-terminal extension that forms an alpha helix in the complex necessary for hierarchical assembly of TERT with p65-TER. This work provides the first structural insight into biogenesis and assembly of TER with a telomerase-specific protein. Additionally, our studies define a structurally homologous domain (xRRM) in genuine La and LARP7 proteins and suggest a general mode of RNA binding for biogenesis of their diverse RNA targets. | ||
| - | + | Structural Basis for Telomerase RNA Recognition and RNP Assembly by the Holoenzyme La Family Protein p65.,Singh M, Wang Z, Koo BK, Patel A, Cascio D, Collins K, Feigon J Mol Cell. 2012 Jun 14. PMID:22705372<ref>PMID:22705372</ref> | |
| - | <ref | + | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | + | </div> | |
| - | [[ | + | <div class="pdbe-citations 4erd" style="background-color:#fffaf0;"></div> |
| - | + | ||
| - | + | ==See Also== | |
| - | [[Category: | + | *[[Telomerase-associated protein|Telomerase-associated protein]] |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Tetrahymena thermophila]] |
| - | [[Category: | + | [[Category: Cascio D]] |
| - | [[Category: | + | [[Category: Collins K]] |
| + | [[Category: Feigon J]] | ||
| + | [[Category: Koo B-K]] | ||
| + | [[Category: Patel A]] | ||
| + | [[Category: Singh M]] | ||
| + | [[Category: Wang Z]] | ||
Current revision
Crystal structure of the C-terminal domain of Tetrahymena telomerase protein p65 in complex with stem IV of telomerase RNA
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Categories: Large Structures | Tetrahymena thermophila | Cascio D | Collins K | Feigon J | Koo B-K | Patel A | Singh M | Wang Z
