4pxu

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{{STRUCTURE_4pxu| PDB=4pxu | SCENE= }}
 
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===Structural basis for the assembly of the mitotic motor kinesin-5 into bipolar tetramers===
 
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{{ABSTRACT_PUBMED_24714498}}
 
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==Function==
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==Structural basis for the assembly of the mitotic motor kinesin-5 into bipolar tetramers==
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[[http://www.uniprot.org/uniprot/KL61_DROME KL61_DROME]] Important role in mitotic dividing cells. Microtubule motor required for spindle body separation. Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function.
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<StructureSection load='4pxu' size='340' side='right'caption='[[4pxu]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4pxu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PXU FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.601&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pxu OCA], [https://pdbe.org/4pxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pxu RCSB], [https://www.ebi.ac.uk/pdbsum/4pxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pxu ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/KL61_DROME KL61_DROME] Important role in mitotic dividing cells. Microtubule motor required for spindle body separation. Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Chromosome segregation during mitosis depends upon Kinesin-5 motors, which display a conserved, bipolar homotetrameric organization consisting of two motor dimers at opposite ends of a central rod. Kinesin-5 motors crosslink adjacent microtubules to drive or constrain their sliding apart, but the structural basis of their organization is unknown. In this study, we report the atomic structure of the bipolar assembly (BASS) domain that directs four Kinesin-5 subunits to form a bipolar minifilament. BASS is a novel 26-nm four-helix bundle, consisting of two anti-parallel coiled-coils at its center, stabilized by alternating hydrophobic and ionic four-helical interfaces, which based on mutagenesis experiments, are critical for tetramerization. Strikingly, N-terminal BASS helices bend as they emerge from the central bundle, swapping partner helices, to form dimeric parallel coiled-coils at both ends, which are offset by 90 degrees . We propose that BASS is a mechanically stable, plectonemically-coiled junction, transmitting forces between Kinesin-5 motor dimers during microtubule sliding. DOI: http://dx.doi.org/10.7554/eLife.02217.001.
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==About this Structure==
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Structural basis for the assembly of the mitotic motor Kinesin-5 into bipolar tetramers.,Scholey JE, Nithianantham S, Scholey JM, Al-Bassam J Elife. 2014 Apr 8;3:e02217. doi: 10.7554/eLife.02217. PMID:24714498<ref>PMID:24714498</ref>
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[[4pxu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PXU OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<ref group="xtra">PMID:024714498</ref><references group="xtra"/><references/>
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</div>
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[[Category: Al-Bassam, J.]]
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<div class="pdbe-citations 4pxu" style="background-color:#fffaf0;"></div>
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[[Category: Nithianantham, S.]]
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[[Category: Scholey, J E.]]
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==See Also==
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[[Category: Scholey, J M.]]
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*[[Kinesin 3D Structures|Kinesin 3D Structures]]
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[[Category: Anti-parallel four-helix bundle]]
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== References ==
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[[Category: Bipolar assembly domain of kinesin-5]]
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<references/>
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[[Category: Bipolar tetramer]]
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__TOC__
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[[Category: Cell cycle]]
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</StructureSection>
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[[Category: Coiled-coil]]
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[[Category: Drosophila melanogaster]]
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[[Category: Crosslinking adjacent microtubules into bundles throughout the mitotic spindle]]
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[[Category: Large Structures]]
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[[Category: Kinesin-5 functions via a sliding filament mechanism]]
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[[Category: Al-Bassam J]]
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[[Category: Microtubule]]
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[[Category: Nithianantham S]]
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[[Category: Scholey JE]]
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[[Category: Scholey JM]]

Current revision

Structural basis for the assembly of the mitotic motor kinesin-5 into bipolar tetramers

PDB ID 4pxu

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