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3wq4

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{{STRUCTURE_3wq4| PDB=3wq4 | SCENE= }}
 
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===Crystal structure of beta-primeverosidase===
 
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==About this Structure==
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==Crystal structure of beta-primeverosidase==
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[[3wq4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQ4 OCA].
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<StructureSection load='3wq4' size='340' side='right'caption='[[3wq4]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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[[Category: Beta-primeverosidase]]
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== Structural highlights ==
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[[Category: Saino, H.]]
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<table><tr><td colspan='2'>[[3wq4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camellia_sinensis Camellia sinensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WQ4 FirstGlance]. <br>
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[[Category: Aroma formation]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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[[Category: Black tea]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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[[Category: Diglycosidase]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wq4 OCA], [https://pdbe.org/3wq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wq4 RCSB], [https://www.ebi.ac.uk/pdbsum/3wq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wq4 ProSAT]</span></td></tr>
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[[Category: Diglycoside]]
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</table>
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[[Category: Disaccharide]]
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== Function ==
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[[Category: Hydrolase]]
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[https://www.uniprot.org/uniprot/Q7X9A9_CAMSI Q7X9A9_CAMSI]
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[[Category: Oolong tea]]
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<div style="background-color:#fffaf0;">
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[[Category: Specific hydrolysis of beta-primeveroside]]
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== Publication Abstract from PubMed ==
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beta-Primeverosidase (PD) is a disaccharide-specific beta-glycosidase in tea leaves. This enzyme is involved in aroma formation during the manufacturing process of oolong tea and black tea. PD hydrolyzes beta-primeveroside (6-O-beta-d-xylopyranosyl-beta-d-glucopyranoside) at the beta-glycosidic bond of primeverose to aglycone, and releases aromatic alcoholic volatiles of aglycones. PD only accepts primeverose as the glycone substrate, but broadly accepts various aglycones, including 2-phenylethanol, benzyl alcohol, linalool, and geraniol. We determined the crystal structure of PD complexes using highly specific disaccharide amidine inhibitors, N-beta-primeverosylamidines, and revealed the architecture of the active site responsible for substrate specificity. We identified three subsites in the active site: subsite -2 specific for 6-O-beta-d-xylopyranosyl, subsite -1 well conserved among beta-glucosidases and specific for beta-d-glucopyranosyl, and wide subsite +1 for hydrophobic aglycone. Glu-470, Ser-473, and Gln-477 act as the specific hydrogen bond donors for 6-O-beta-d-xylopyranosyl in subsite -2. On the other hand, subsite +1 was a large hydrophobic cavity that accommodates various aromatic aglycones. Compared with aglycone-specific beta-glucosidases of the glycoside hydrolase family 1, PD lacks the Trp crucial for aglycone recognition, and the resultant large cavity accepts aglycone and 6-O-beta-d-xylopyranosyl together. PD recognizes the beta-primeverosides in subsites -1 and -2 by hydrogen bonds, whereas the large subsite +1 loosely accommodates various aglycones. The glycone-specific activity of PD for broad aglycone substrates results in selective and multiple release of temporally stored alcoholic volatile aglycones of beta-primeveroside.
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Crystal structures of beta-primeverosidase in complex with disaccharide amidine inhibitors.,Saino H, Shimizu T, Hiratake J, Nakatsu T, Kato H, Sakata K, Mizutani M J Biol Chem. 2014 Jun 13;289(24):16826-34. doi: 10.1074/jbc.M114.553271. Epub, 2014 Apr 21. PMID:24753293<ref>PMID:24753293</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3wq4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Camellia sinensis]]
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[[Category: Large Structures]]
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[[Category: Saino H]]

Current revision

Crystal structure of beta-primeverosidase

PDB ID 3wq4

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