4cqo

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{{STRUCTURE_4cqo| PDB=4cqo | SCENE= }}
 
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===Structure of the human CNOT1 superfamily homology domain in complex with a Nanos1 peptide===
 
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==Disease==
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==Structure of the human CNOT1 superfamily homology domain in complex with a Nanos1 peptide==
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[[http://www.uniprot.org/uniprot/NANO1_HUMAN NANO1_HUMAN]] Male infertility due to NANOS1 mutation. The disease is caused by mutations affecting the gene represented in this entry.
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<StructureSection load='4cqo' size='340' side='right'caption='[[4cqo]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4cqo]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CQO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CQO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cqo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cqo OCA], [https://pdbe.org/4cqo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cqo RCSB], [https://www.ebi.ac.uk/pdbsum/4cqo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cqo ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CNOT1_HUMAN CNOT1_HUMAN] Belongs to the CCR4-NOT complex that functions as general transcription regulation complex. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors.<ref>PMID:10637334</ref> <ref>PMID:16778766</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The RNA-binding proteins of the Nanos family play an essential role in germ cell development and survival in a wide range of metazoan species. They function by suppressing the expression of target mRNAs through the recruitment of effector complexes, which include the CCR4-NOT deadenylase complex. Here, we show that the three human Nanos paralogs (Nanos1-3) interact with the CNOT1 C-terminal domain and determine the structural basis for the specific molecular recognition. Nanos1-3 bind CNOT1 through a short CNOT1-interacting motif (NIM) that is conserved in all vertebrates and some invertebrate species. The crystal structure of the human Nanos1 NIM peptide bound to CNOT1 reveals that the peptide opens a conserved hydrophobic pocket on the CNOT1 surface by inserting conserved aromatic residues. The substitutions of these aromatic residues in the Nanos1-3 NIMs abolish binding to CNOT1 and abrogate the ability of the proteins to repress translation. Our findings provide the structural basis for the recruitment of the CCR4-NOT complex by vertebrate Nanos, indicate that the NIMs are the major determinants of the translational repression mediated by Nanos, and identify the CCR4-NOT complex as the main effector complex for Nanos function.
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==Function==
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Structural basis for the Nanos-mediated recruitment of the CCR4-NOT complex and translational repression.,Bhandari D, Raisch T, Weichenrieder O, Jonas S, Izaurralde E Genes Dev. 2014 Apr 15;28(8):888-901. doi: 10.1101/gad.237289.113. PMID:24736845<ref>PMID:24736845</ref>
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[[http://www.uniprot.org/uniprot/CNOT1_HUMAN CNOT1_HUMAN]] Belongs to the CCR4-NOT complex that functions as general transcription regulation complex. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors.<ref>PMID:10637334</ref> <ref>PMID:16778766</ref> [[http://www.uniprot.org/uniprot/NANO1_HUMAN NANO1_HUMAN]] May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.<ref>PMID:17047063</ref> <ref>PMID:18223680</ref>
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[4cqo]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CQO OCA].
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</div>
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<div class="pdbe-citations 4cqo" style="background-color:#fffaf0;"></div>
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==Reference==
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== References ==
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<references group="xtra"/><references/>
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<references/>
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[[Category: Bhandari, D.]]
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__TOC__
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[[Category: Izaurralde, E.]]
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</StructureSection>
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[[Category: Jonas, S.]]
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[[Category: Homo sapiens]]
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[[Category: Raisch, T.]]
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[[Category: Large Structures]]
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[[Category: Weichenrieder, O.]]
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[[Category: Bhandari D]]
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[[Category: Deadenylation]]
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[[Category: Izaurralde E]]
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[[Category: Development]]
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[[Category: Jonas S]]
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[[Category: Gene regulation]]
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[[Category: Raisch T]]
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[[Category: Protein complex]]
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[[Category: Weichenrieder O]]
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[[Category: Short linear motif]]
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[[Category: Translation]]
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[[Category: Translational repression]]
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Current revision

Structure of the human CNOT1 superfamily homology domain in complex with a Nanos1 peptide

PDB ID 4cqo

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