2b9s
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| - | {{STRUCTURE_2b9s| PDB=2b9s | SCENE= }} | ||
| - | ===Crystal Structure of heterodimeric L. donovani topoisomerase I-vanadate-DNA complex=== | ||
| - | {{ABSTRACT_PUBMED_16487540}} | ||
| - | == | + | ==Crystal Structure of heterodimeric L. donovani topoisomerase I-vanadate-DNA complex== |
| - | [[2b9s]] is a 5 chain structure with sequence from [ | + | <StructureSection load='2b9s' size='340' side='right'caption='[[2b9s]], [[Resolution|resolution]] 2.27Å' scene=''> |
| - | + | == Structural highlights == | |
| - | == | + | <table><tr><td colspan='2'>[[2b9s]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_donovani Leishmania donovani]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B9S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B9S FirstGlance]. <br> |
| - | < | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27Å</td></tr> |
| - | [[ | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VO4:VANADATE+ION'>VO4</scene></td></tr> |
| - | [[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b9s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b9s OCA], [https://pdbe.org/2b9s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b9s RCSB], [https://www.ebi.ac.uk/pdbsum/2b9s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b9s ProSAT]</span></td></tr> |
| - | [ | + | </table> |
| - | [[ | + | == Function == |
| - | [[Category: | + | [https://www.uniprot.org/uniprot/Q9GPZ9_LEIIN Q9GPZ9_LEIIN] Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.[RuleBase:RU365101] |
| - | [[Category: | + | == Evolutionary Conservation == |
| - | [[Category: | + | [[Image:Consurf_key_small.gif|200px|right]] |
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/2b9s_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b9s ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Leishmania donovani]] | ||
| + | [[Category: Davies DR]] | ||
| + | [[Category: Hol WGJ]] | ||
Current revision
Crystal Structure of heterodimeric L. donovani topoisomerase I-vanadate-DNA complex
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