2cnb

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{{STRUCTURE_2cnb| PDB=2cnb | SCENE= }}
 
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===TRYPANOSOMA BRUCEI UDP-GALACTOSE-4-EPIMERASE IN TERNARY COMPLEX WITH NAD AND THE SUBSTRATE ANALOGUE UDP-4-DEOXY-4-FLUORO-ALPHA-D-GALACTOSE===
 
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{{ABSTRACT_PUBMED_16946458}}
 
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==About this Structure==
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==Trypanosoma brucei UDP-galactose-4-epimerase in ternary complex with NAD and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose==
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[[2cnb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNB OCA].
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<StructureSection load='2cnb' size='340' side='right'caption='[[2cnb]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2cnb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CNB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=UFG:URIDINE-5-DIPHOSPHATE-4-DEOXY-4-FLUORO-ALPHA-D-GALACTOSE'>UFG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cnb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cnb OCA], [https://pdbe.org/2cnb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cnb RCSB], [https://www.ebi.ac.uk/pdbsum/2cnb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cnb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8T8E9_9TRYP Q8T8E9_9TRYP]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/2cnb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cnb ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The structure of the NAD-dependent oxidoreductase UDP-galactose-4'-epimerase from Trypanosoma brucei in complex with cofactor and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose has been determined using diffraction data to 2.7 A resolution. Despite the high level of sequence and structure conservation between the trypanosomatid enzyme and those from humans, yeast and bacteria, the binding of the 4-fluoro-alpha-D-galactose moiety is distinct from previously reported structures. Of particular note is the observation that when bound to the T. brucei enzyme, the galactose moiety of this fluoro-derivative is rotated approximately 180 degrees with respect to the orientation of the hexose component of UDP-glucose when in complex with the human enzyme. The architecture of the catalytic centre is designed to effectively bind different orientations of the hexose, a finding that is consistent with a mechanism that requires the sugar to maintain a degree of flexibility within the active site.
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Trypanosoma brucei UDP-galactose-4'-epimerase in ternary complex with NAD+ and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose.,Alphey MS, Burton A, Urbaniak MD, Boons GJ, Ferguson MA, Hunter WN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):829-34. Epub 2006 Aug 11. PMID:16946458<ref>PMID:16946458</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2cnb" style="background-color:#fffaf0;"></div>
==See Also==
==See Also==
*[[UDP-galactose 4-epimerase|UDP-galactose 4-epimerase]]
*[[UDP-galactose 4-epimerase|UDP-galactose 4-epimerase]]
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*[[UDP-galactose 4-epimerase 3D structures|UDP-galactose 4-epimerase 3D structures]]
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==Reference==
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== References ==
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<ref group="xtra">PMID:016946458</ref><references group="xtra"/><references/>
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<references/>
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[[Category: Trypanosoma brucei]]
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__TOC__
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[[Category: UDP-glucose 4-epimerase]]
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</StructureSection>
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[[Category: Alphey, M S.]]
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[[Category: Large Structures]]
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[[Category: Ferguson, M A.J.]]
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[[Category: Hunter, W N.]]
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[[Category: Epimerase]]
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[[Category: Isomerase]]
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[[Category: Nad]]
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[[Category: Short-chain dehydrogenase/reductase]]
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[[Category: Trypanosoma brucei]]
[[Category: Trypanosoma brucei]]
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[[Category: Udp-4-deoxy- 4-fluoro-alpha-d-galactose]]
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[[Category: Alphey MS]]
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[[Category: Udp-galactose-4- epimerase]]
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[[Category: Ferguson MAJ]]
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[[Category: Hunter WN]]

Current revision

Trypanosoma brucei UDP-galactose-4-epimerase in ternary complex with NAD and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose

PDB ID 2cnb

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