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2cnb
From Proteopedia
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| - | {{STRUCTURE_2cnb| PDB=2cnb | SCENE= }} | ||
| - | ===TRYPANOSOMA BRUCEI UDP-GALACTOSE-4-EPIMERASE IN TERNARY COMPLEX WITH NAD AND THE SUBSTRATE ANALOGUE UDP-4-DEOXY-4-FLUORO-ALPHA-D-GALACTOSE=== | ||
| - | {{ABSTRACT_PUBMED_16946458}} | ||
| - | == | + | ==Trypanosoma brucei UDP-galactose-4-epimerase in ternary complex with NAD and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose== |
| - | [[2cnb]] is a 4 chain structure with sequence from [ | + | <StructureSection load='2cnb' size='340' side='right'caption='[[2cnb]], [[Resolution|resolution]] 2.70Å' scene=''> |
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[2cnb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei Trypanosoma brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CNB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CNB FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=UFG:URIDINE-5-DIPHOSPHATE-4-DEOXY-4-FLUORO-ALPHA-D-GALACTOSE'>UFG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cnb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cnb OCA], [https://pdbe.org/2cnb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cnb RCSB], [https://www.ebi.ac.uk/pdbsum/2cnb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cnb ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q8T8E9_9TRYP Q8T8E9_9TRYP] | ||
| + | == Evolutionary Conservation == | ||
| + | [[Image:Consurf_key_small.gif|200px|right]] | ||
| + | Check<jmol> | ||
| + | <jmolCheckbox> | ||
| + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/2cnb_consurf.spt"</scriptWhenChecked> | ||
| + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
| + | <text>to colour the structure by Evolutionary Conservation</text> | ||
| + | </jmolCheckbox> | ||
| + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cnb ConSurf]. | ||
| + | <div style="clear:both"></div> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The structure of the NAD-dependent oxidoreductase UDP-galactose-4'-epimerase from Trypanosoma brucei in complex with cofactor and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose has been determined using diffraction data to 2.7 A resolution. Despite the high level of sequence and structure conservation between the trypanosomatid enzyme and those from humans, yeast and bacteria, the binding of the 4-fluoro-alpha-D-galactose moiety is distinct from previously reported structures. Of particular note is the observation that when bound to the T. brucei enzyme, the galactose moiety of this fluoro-derivative is rotated approximately 180 degrees with respect to the orientation of the hexose component of UDP-glucose when in complex with the human enzyme. The architecture of the catalytic centre is designed to effectively bind different orientations of the hexose, a finding that is consistent with a mechanism that requires the sugar to maintain a degree of flexibility within the active site. | ||
| + | |||
| + | Trypanosoma brucei UDP-galactose-4'-epimerase in ternary complex with NAD+ and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose.,Alphey MS, Burton A, Urbaniak MD, Boons GJ, Ferguson MA, Hunter WN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):829-34. Epub 2006 Aug 11. PMID:16946458<ref>PMID:16946458</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 2cnb" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
*[[UDP-galactose 4-epimerase|UDP-galactose 4-epimerase]] | *[[UDP-galactose 4-epimerase|UDP-galactose 4-epimerase]] | ||
| - | + | *[[UDP-galactose 4-epimerase 3D structures|UDP-galactose 4-epimerase 3D structures]] | |
| - | == | + | == References == |
| - | + | <references/> | |
| - | [[Category: | + | __TOC__ |
| - | + | </StructureSection> | |
| - | + | [[Category: Large Structures]] | |
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[[Category: Trypanosoma brucei]] | [[Category: Trypanosoma brucei]] | ||
| - | [[Category: | + | [[Category: Alphey MS]] |
| - | [[Category: | + | [[Category: Ferguson MAJ]] |
| + | [[Category: Hunter WN]] | ||
Current revision
Trypanosoma brucei UDP-galactose-4-epimerase in ternary complex with NAD and the substrate analogue UDP-4-deoxy-4-fluoro-alpha-D-galactose
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