2p3k

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(New page: 200px<br /><applet load="2p3k" size="350" color="white" frame="true" align="right" spinBox="true" caption="2p3k, resolution 1.56&Aring;" /> '''Crystal structure of...)
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[[Image:2p3k.jpg|left|200px]]<br /><applet load="2p3k" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2p3k, resolution 1.56&Aring;" />
 
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'''Crystal structure of Rhesus rotavirus VP8* at 100K'''<br />
 
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==About this Structure==
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==Crystal structure of Rhesus rotavirus VP8* at 100K==
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2P3K is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rhesus_rotavirus Rhesus rotavirus] with <scene name='pdbligand=SO4:'>SO4</scene>, <scene name='pdbligand=MNA:'>MNA</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Sites: <scene name='pdbsite=AC1:So4+Binding+Site+For+Residue+A+3000'>AC1</scene>, <scene name='pdbsite=AC2:Mna+Binding+Site+For+Residue+A+1000'>AC2</scene> and <scene name='pdbsite=AC3:Gol+Binding+Site+For+Residue+A+4000'>AC3</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P3K OCA].
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<StructureSection load='2p3k' size='340' side='right'caption='[[2p3k]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
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[[Category: Rhesus rotavirus]]
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== Structural highlights ==
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[[Category: Single protein]]
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<table><tr><td colspan='2'>[[2p3k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_rotavirus_A_strain_RRV Simian rotavirus A strain RRV]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P3K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P3K FirstGlance]. <br>
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[[Category: Blanchard, H.]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
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[[Category: GOL]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MNA:2-O-METHYL-5-N-ACETYL-ALPHA-D-+NEURAMINIC+ACID'>MNA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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[[Category: MNA]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p3k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p3k OCA], [https://pdbe.org/2p3k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p3k RCSB], [https://www.ebi.ac.uk/pdbsum/2p3k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p3k ProSAT]</span></td></tr>
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[[Category: SO4]]
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</table>
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[[Category: beta-sandwich]]
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== Function ==
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[[Category: viral protein]]
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[https://www.uniprot.org/uniprot/VP4_ROTRH VP4_ROTRH] Spike-forming protein that mediates virion attachment to the host epithelial cell receptors and plays a major role in cell penetration, determination of host range restriction and virulence. It is subsequently lost, together with VP7, following virus entry into the host cell. Rotavirus attachment and entry into the host cell probably involves multiple sequential contacts between the outer capsid proteins VP4 and VP7, and the cell receptors. In sialic acid-dependent and/or integrin-dependent strains, VP4 seems to essentially target sialic acid and/or the integrin heterodimer ITGA2/ITGB1.<ref>PMID:20375171</ref> Outer capsid protein VP5*: forms the spike "foot" and "body". Acts as a membrane permeabilization protein that mediates release of viral particles from endosomal compartments into the cytoplasm. In integrin-dependent strains, VP5* targets the integrin heterodimer ITGA2/ITGB1 for cell attachment.<ref>PMID:20375171</ref> VP8* forms the head of the spikes. It is the viral hemagglutinin and an important target of neutralizing antibodies. In sialic acid-dependent strains, VP8* binds to host cell sialic acid, most probably a ganglioside, providing the initial contact.<ref>PMID:20375171</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/p3/2p3k_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2p3k ConSurf].
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<div style="clear:both"></div>
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Mar 14 09:32:28 2008''
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==See Also==
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Simian rotavirus A strain RRV]]
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[[Category: Blanchard H]]

Current revision

Crystal structure of Rhesus rotavirus VP8* at 100K

PDB ID 2p3k

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