2ler
From Proteopedia
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==Conotoxin pc16a== | ==Conotoxin pc16a== | ||
| - | <StructureSection load='2ler' size='340' side='right' caption='[[2ler | + | <StructureSection load='2ler' size='340' side='right'caption='[[2ler]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | [[2ler]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LER OCA]. <br> | + | <table><tr><td colspan='2'>[[2ler]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_pictus Conus pictus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LER OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LER FirstGlance]. <br> |
| - | <b> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ler FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ler OCA], [https://pdbe.org/2ler PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ler RCSB], [https://www.ebi.ac.uk/pdbsum/2ler PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ler ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
A novel conotoxin, pc16a, was isolated from the venom of Conus pictus. This is the first peptide characterized from this South-African cone snail and it has only 11 amino acid residues, SCSCKRNFLCC*, with the rare cysteine framework XVI and a monoisotopic mass of 1257.6Da. Two peptides were synthesized with two possible conformations: globular (pc16a_1) and ribbon (pc16a_2). pc16a_1 co-eluted with the native peptide, which indicates a disulfide connectivity I-III, II-IV. The structure of pc16a_1 was determined by NMR. Both synthetic peptides were used to elucidate the biological activity. Bioassays were performed on crickets, ghost shrimps, larvae of the mealworm beetle and mice, but no effect was seen. Using two-electrode voltage clamp, a range of voltage-gated ion channels (Na(v) and K(v)) and nicotinic acetylcholine receptors were screened, but again no activity was found. Hence, the specific target of pc16a still remains to be discovered. | A novel conotoxin, pc16a, was isolated from the venom of Conus pictus. This is the first peptide characterized from this South-African cone snail and it has only 11 amino acid residues, SCSCKRNFLCC*, with the rare cysteine framework XVI and a monoisotopic mass of 1257.6Da. Two peptides were synthesized with two possible conformations: globular (pc16a_1) and ribbon (pc16a_2). pc16a_1 co-eluted with the native peptide, which indicates a disulfide connectivity I-III, II-IV. The structure of pc16a_1 was determined by NMR. Both synthetic peptides were used to elucidate the biological activity. Bioassays were performed on crickets, ghost shrimps, larvae of the mealworm beetle and mice, but no effect was seen. Using two-electrode voltage clamp, a range of voltage-gated ion channels (Na(v) and K(v)) and nicotinic acetylcholine receptors were screened, but again no activity was found. Hence, the specific target of pc16a still remains to be discovered. | ||
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Pc16a, the first characterized peptide from Conus pictus venom, shows a novel disulfide connectivity.,Van Der Haegen A, Peigneur S, Dyubankova N, Moller C, Mari F, Diego-Garcia E, Naude R, Lescrinier E, Herdewijn P, Tytgat J Peptides. 2012 Mar;34(1):106-13. Epub 2011 Nov 3. PMID:22079220<ref>PMID:22079220</ref> | Pc16a, the first characterized peptide from Conus pictus venom, shows a novel disulfide connectivity.,Van Der Haegen A, Peigneur S, Dyubankova N, Moller C, Mari F, Diego-Garcia E, Naude R, Lescrinier E, Herdewijn P, Tytgat J Peptides. 2012 Mar;34(1):106-13. Epub 2011 Nov 3. PMID:22079220<ref>PMID:22079220</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| + | </div> | ||
| + | <div class="pdbe-citations 2ler" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Conus pictus]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: Lescrinier | + | [[Category: Dyubankova N]] |
| - | [[Category: Peigneur | + | [[Category: Lescrinier E]] |
| - | [[Category: Tytgat | + | [[Category: Peigneur S]] |
| - | [[Category: | + | [[Category: Tytgat J]] |
| + | [[Category: Van Der Haegen A]] | ||
Current revision
Conotoxin pc16a
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