2l1o
From Proteopedia
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==Zinc to cadmium replacement in the A. thaliana SUPERMAN Cys2His2 zinc finger induces structural rearrangements of typical DNA base determinant positions== | ==Zinc to cadmium replacement in the A. thaliana SUPERMAN Cys2His2 zinc finger induces structural rearrangements of typical DNA base determinant positions== | ||
| - | <StructureSection load='2l1o' size='340' side='right' caption='[[2l1o | + | <StructureSection load='2l1o' size='340' side='right'caption='[[2l1o]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | [[2l1o]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L1O OCA]. <br> | + | <table><tr><td colspan='2'>[[2l1o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L1O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L1O FirstGlance]. <br> |
| - | <b> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l1o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l1o OCA], [https://pdbe.org/2l1o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l1o RCSB], [https://www.ebi.ac.uk/pdbsum/2l1o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l1o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SUP_ARATH SUP_ARATH] Probable transcriptional regulator considered as cadastral protein that acts indirectly to prevent the B class homeotic proteins APETALA3 and perhaps PISTILLATA from acting in the gynoecial whorl. Principal function is to balance cell proliferation in the third and fourth whorls of developing flowers thereby maintaining the boundary between stamens and carpels. May fulfill this role by repressing genes implicated in cell division. Plays equally a role in the determinacy of the floral meristem. Is also required for normal ovule development.<ref>PMID:7477325</ref> <ref>PMID:12324589</ref> <ref>PMID:1352237</ref> <ref>PMID:11006335</ref> <ref>PMID:11943180</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
Among heavy metals, whose toxicity cause a steadily increasing of environmental pollution, cadmium is of special concern due to its relatively high mobility in soils and potential toxicity at low concentrations. Given their ubiquitous role, zinc fingers domains have been proposed as mediators for the toxic and carcinogenic effects exerted by xenobiotic metals. To verify the structural effects of zinc replacement by cadmium in zinc fingers we have determined the high resolution structure of the single Cys(2) His(2) zinc finger of the A. thaliana Superman protein (SUP37) complexed to the cadmium ion by means of UV-Vis and NMR techniques. SUP37 is able to bind Cd(II) though with a dissociation constant higher than that measured for Zn(II). Cd-SUP37 retains the betabetaalpha fold but experiences a global structural rearrangement affecting both the relative orientation of the secondary structure elements and the position of side chains involved in DNA recognition: among them Ser17 side chain, which we show to be essential for DNA binding, experiences the largest displacement. (c) 2011 Wiley Periodicals, Inc. Biopolymers, 2011. | Among heavy metals, whose toxicity cause a steadily increasing of environmental pollution, cadmium is of special concern due to its relatively high mobility in soils and potential toxicity at low concentrations. Given their ubiquitous role, zinc fingers domains have been proposed as mediators for the toxic and carcinogenic effects exerted by xenobiotic metals. To verify the structural effects of zinc replacement by cadmium in zinc fingers we have determined the high resolution structure of the single Cys(2) His(2) zinc finger of the A. thaliana Superman protein (SUP37) complexed to the cadmium ion by means of UV-Vis and NMR techniques. SUP37 is able to bind Cd(II) though with a dissociation constant higher than that measured for Zn(II). Cd-SUP37 retains the betabetaalpha fold but experiences a global structural rearrangement affecting both the relative orientation of the secondary structure elements and the position of side chains involved in DNA recognition: among them Ser17 side chain, which we show to be essential for DNA binding, experiences the largest displacement. (c) 2011 Wiley Periodicals, Inc. Biopolymers, 2011. | ||
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Zinc to cadmium replacement in the A. thaliana SUPERMAN Cys(2) His(2) zinc finger induces structural rearrangements of typical DNA base determinant positions.,Malgieri G, Zaccaro L, Leone M, Bucci E, Esposito S, Baglivo I, Del Gatto A, Russo L, Scandurra R, Pedone PV, Fattorusso R, Isernia C Biopolymers. 2011 May 25. doi: 10.1002/bip.21680. PMID:21618209<ref>PMID:21618209</ref> | Zinc to cadmium replacement in the A. thaliana SUPERMAN Cys(2) His(2) zinc finger induces structural rearrangements of typical DNA base determinant positions.,Malgieri G, Zaccaro L, Leone M, Bucci E, Esposito S, Baglivo I, Del Gatto A, Russo L, Scandurra R, Pedone PV, Fattorusso R, Isernia C Biopolymers. 2011 May 25. doi: 10.1002/bip.21680. PMID:21618209<ref>PMID:21618209</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| + | </div> | ||
| + | <div class="pdbe-citations 2l1o" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Baglivo I]] |
| - | [[Category: | + | [[Category: Bucci E]] |
| - | [[Category: Gatto | + | [[Category: Del Gatto A]] |
| - | [[Category: | + | [[Category: Esposito S]] |
| - | [[Category: | + | [[Category: Fattorusso R]] |
| - | [[Category: | + | [[Category: Isernia C]] |
| - | [[Category: | + | [[Category: Leone M]] |
| - | [[Category: | + | [[Category: Malgieri G]] |
| - | [[Category: | + | [[Category: Pedone PV]] |
| - | [[Category: | + | [[Category: Scandurra R]] |
| - | [[Category: | + | [[Category: Zaccaro L]] |
| - | + | ||
| - | + | ||
Current revision
Zinc to cadmium replacement in the A. thaliana SUPERMAN Cys2His2 zinc finger induces structural rearrangements of typical DNA base determinant positions
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Categories: Arabidopsis thaliana | Large Structures | Baglivo I | Bucci E | Del Gatto A | Esposito S | Fattorusso R | Isernia C | Leone M | Malgieri G | Pedone PV | Scandurra R | Zaccaro L
