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2lkr
From Proteopedia
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==Yeast U2/U6 complex== | ==Yeast U2/U6 complex== | ||
| - | <StructureSection load='2lkr' size='340' side='right' caption='[[2lkr | + | <StructureSection load='2lkr' size='340' side='right'caption='[[2lkr]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | [[2lkr]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2lkr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LKR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LKR FirstGlance]. <br> |
| - | <b> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <b> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lkr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lkr OCA], [https://pdbe.org/2lkr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lkr RCSB], [https://www.ebi.ac.uk/pdbsum/2lkr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lkr ProSAT]</span></td></tr> |
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
The U2/U6 snRNA complex is a conserved and essential component of the active spliceosome that interacts with the pre-mRNA substrate and essential protein splicing factors to promote splicing catalysis. Here we have elucidated the solution structure of a 111-nucleotide U2/U6 complex using an approach that integrates SAXS, NMR, and molecular modeling. The U2/U6 structure contains a three-helix junction that forms an extended "Y" shape. The U6 internal stem-loop (ISL) forms a continuous stack with U2/U6 Helices Ib, Ia, and III. The coaxial stacking of Helix Ib on the U6 ISL is a configuration that is similar to the Domain V structure in group II introns. Interestingly, essential features of the complex-including the U80 metal binding site, AGC triad, and pre-mRNA recognition sites-localize to one face of the molecule. This observation suggests that the U2/U6 structure is well-suited for orienting substrate and cofactors during splicing catalysis. | The U2/U6 snRNA complex is a conserved and essential component of the active spliceosome that interacts with the pre-mRNA substrate and essential protein splicing factors to promote splicing catalysis. Here we have elucidated the solution structure of a 111-nucleotide U2/U6 complex using an approach that integrates SAXS, NMR, and molecular modeling. The U2/U6 structure contains a three-helix junction that forms an extended "Y" shape. The U6 internal stem-loop (ISL) forms a continuous stack with U2/U6 Helices Ib, Ia, and III. The coaxial stacking of Helix Ib on the U6 ISL is a configuration that is similar to the Domain V structure in group II introns. Interestingly, essential features of the complex-including the U80 metal binding site, AGC triad, and pre-mRNA recognition sites-localize to one face of the molecule. This observation suggests that the U2/U6 structure is well-suited for orienting substrate and cofactors during splicing catalysis. | ||
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Structure of the yeast U2/U6 snRNA complex.,Burke JE, Sashital DG, Zuo X, Wang YX, Butcher SE RNA. 2012 Feb 10. PMID:22328579<ref>PMID:22328579</ref> | Structure of the yeast U2/U6 snRNA complex.,Burke JE, Sashital DG, Zuo X, Wang YX, Butcher SE RNA. 2012 Feb 10. PMID:22328579<ref>PMID:22328579</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| + | </div> | ||
| + | <div class="pdbe-citations 2lkr" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Saccharomyces cerevisiae]] | [[Category: Saccharomyces cerevisiae]] | ||
| - | [[Category: Burke | + | [[Category: Burke JE]] |
| - | [[Category: Butcher | + | [[Category: Butcher SE]] |
| - | [[Category: Sashital | + | [[Category: Sashital DG]] |
| - | [[Category: Wang | + | [[Category: Wang Y]] |
| - | [[Category: Zuo | + | [[Category: Zuo X]] |
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Current revision
Yeast U2/U6 complex
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