2lc8
From Proteopedia
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==Solution structure of the MLV readthrough pseudoknot== | ==Solution structure of the MLV readthrough pseudoknot== | ||
| - | <StructureSection load='2lc8' size='340' side='right' caption='[[2lc8 | + | <StructureSection load='2lc8' size='340' side='right'caption='[[2lc8]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | [[2lc8]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LC8 OCA]. <br> | + | <table><tr><td colspan='2'>[[2lc8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LC8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LC8 FirstGlance]. <br> |
| - | <b> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lc8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lc8 OCA], [https://pdbe.org/2lc8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lc8 RCSB], [https://www.ebi.ac.uk/pdbsum/2lc8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lc8 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
Most retroviruses require translational recoding of a viral messenger RNA stop codon to maintain a precise ratio of structural (Gag) and enzymatic (Pol) proteins during virus assembly. Pol is expressed exclusively as a Gag-Pol fusion either by ribosomal frameshifting or by read-through of the gag stop codon. Both of these mechanisms occur infrequently and only affect 5-10% of translating ribosomes, allowing the virus to maintain the critical Gag to Gag-Pol ratio. Although it is understood that the frequency of the recoding event is regulated by cis RNA motifs, no mechanistic explanation is currently available for how the critical protein ratio is maintained. Here we present the NMR structure of the murine leukaemia virus recoding signal and show that a protonation-dependent switch occurs to induce the active conformation. The equilibrium is such that at physiological pH the active, read-through permissive conformation is populated at approximately 6%: a level that correlates with in vivo protein quantities. The RNA functions by a highly sensitive, chemo-mechanical coupling tuned to ensure an optimal read-through frequency. Similar observations for a frameshifting signal indicate that this novel equilibrium-based mechanism may have a general role in translational recoding. | Most retroviruses require translational recoding of a viral messenger RNA stop codon to maintain a precise ratio of structural (Gag) and enzymatic (Pol) proteins during virus assembly. Pol is expressed exclusively as a Gag-Pol fusion either by ribosomal frameshifting or by read-through of the gag stop codon. Both of these mechanisms occur infrequently and only affect 5-10% of translating ribosomes, allowing the virus to maintain the critical Gag to Gag-Pol ratio. Although it is understood that the frequency of the recoding event is regulated by cis RNA motifs, no mechanistic explanation is currently available for how the critical protein ratio is maintained. Here we present the NMR structure of the murine leukaemia virus recoding signal and show that a protonation-dependent switch occurs to induce the active conformation. The equilibrium is such that at physiological pH the active, read-through permissive conformation is populated at approximately 6%: a level that correlates with in vivo protein quantities. The RNA functions by a highly sensitive, chemo-mechanical coupling tuned to ensure an optimal read-through frequency. Similar observations for a frameshifting signal indicate that this novel equilibrium-based mechanism may have a general role in translational recoding. | ||
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An equilibrium-dependent retroviral mRNA switch regulates translational recoding.,Houck-Loomis B, Durney MA, Salguero C, Shankar N, Nagle JM, Goff SP, D'Souza VM Nature. 2011 Nov 27;480(7378):561-4. doi: 10.1038/nature10657. PMID:22121021<ref>PMID:22121021</ref> | An equilibrium-dependent retroviral mRNA switch regulates translational recoding.,Houck-Loomis B, Durney MA, Salguero C, Shankar N, Nagle JM, Goff SP, D'Souza VM Nature. 2011 Nov 27;480(7378):561-4. doi: 10.1038/nature10657. PMID:22121021<ref>PMID:22121021</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| + | </div> | ||
| + | <div class="pdbe-citations 2lc8" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Large Structures]] |
| - | [[Category: | + | [[Category: Moloney murine leukemia virus]] |
| - | [[Category: | + | [[Category: Durney MA]] |
| - | [[Category: | + | [[Category: Houck-Loomis B]] |
| - | + | ||
Current revision
Solution structure of the MLV readthrough pseudoknot
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