2l2r
From Proteopedia
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==Helical hairpin structure of a novel antimicrobial peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)== | ==Helical hairpin structure of a novel antimicrobial peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)== | ||
| - | <StructureSection load='2l2r' size='340' side='right' caption='[[2l2r | + | <StructureSection load='2l2r' size='340' side='right'caption='[[2l2r]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | [[2l2r]] is a 1 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2l2r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinochloa_crus-galli Echinochloa crus-galli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L2R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L2R FirstGlance]. <br> |
| - | <b> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <b>Resources:</b> <span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l2r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l2r OCA], [https://pdbe.org/2l2r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l2r RCSB], [https://www.ebi.ac.uk/pdbsum/2l2r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l2r ProSAT]</span></td></tr> |
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
This study presents purification, activity characterization, and (1)H NMR study of the novel antifungal peptide EcAMP1 from kernels of barnyard grass Echinochloa crus-galli. The peptide adopts a disulfide-stabilized alpha-helical hairpin structure in aqueous solution and thus represents a novel fold among naturally occurring antimicrobial peptides. Micromolar concentrations of EcAMP1 were shown to inhibit growth of several fungal phytopathogens. Confocal microscopy revealed intensive EcAMP1 binding to the surface of fungal conidia followed by internalization and accumulation in the cytoplasm without disturbance of membrane integrity. Close spatial structure similarity between EcAMP1, the trypsin inhibitor VhTI from seeds of Veronica hederifolia, and some scorpion and cone snail toxins suggests natural elaboration of different functions on a common fold. | This study presents purification, activity characterization, and (1)H NMR study of the novel antifungal peptide EcAMP1 from kernels of barnyard grass Echinochloa crus-galli. The peptide adopts a disulfide-stabilized alpha-helical hairpin structure in aqueous solution and thus represents a novel fold among naturally occurring antimicrobial peptides. Micromolar concentrations of EcAMP1 were shown to inhibit growth of several fungal phytopathogens. Confocal microscopy revealed intensive EcAMP1 binding to the surface of fungal conidia followed by internalization and accumulation in the cytoplasm without disturbance of membrane integrity. Close spatial structure similarity between EcAMP1, the trypsin inhibitor VhTI from seeds of Veronica hederifolia, and some scorpion and cone snail toxins suggests natural elaboration of different functions on a common fold. | ||
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Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli).,Nolde SB, Vassilevski AA, Rogozhin EA, Barinov NA, Balashova TA, Samsonova OV, Baranov YV, Feofanov AV, Egorov TA, Arseniev AS, Grishin EV J Biol Chem. 2011 Jul 15;286(28):25145-53. Epub 2011 May 11. PMID:21561864<ref>PMID:21561864</ref> | Disulfide-stabilized Helical Hairpin Structure and Activity of a Novel Antifungal Peptide EcAMP1 from Seeds of Barnyard Grass (Echinochloa crus-galli).,Nolde SB, Vassilevski AA, Rogozhin EA, Barinov NA, Balashova TA, Samsonova OV, Baranov YV, Feofanov AV, Egorov TA, Arseniev AS, Grishin EV J Biol Chem. 2011 Jul 15;286(28):25145-53. Epub 2011 May 11. PMID:21561864<ref>PMID:21561864</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| + | </div> | ||
| + | <div class="pdbe-citations 2l2r" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Echinochloa crus-galli]] |
| - | [[Category: Arseniev | + | [[Category: Large Structures]] |
| - | [[Category: Balashova | + | [[Category: Arseniev AS]] |
| - | [[Category: Barinov | + | [[Category: Balashova TA]] |
| - | [[Category: Egorov | + | [[Category: Barinov NA]] |
| - | [[Category: Grishin | + | [[Category: Egorov TA]] |
| - | [[Category: Nolde | + | [[Category: Grishin EV]] |
| - | [[Category: Rogozhin | + | [[Category: Nolde SB]] |
| - | [[Category: Vassilevski | + | [[Category: Rogozhin EA]] |
| - | + | [[Category: Vassilevski AA]] | |
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Current revision
Helical hairpin structure of a novel antimicrobial peptide EcAMP1 from seeds of barnyard grass (Echinochloa crus-galli)
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