JMS/Sandbox21
From Proteopedia
(Difference between revisions)
| (19 intermediate revisions not shown.) | |||
| Line 1: | Line 1: | ||
| - | == | + | ==PKA and Regulators== |
| - | <StructureSection load='3fhi' size=' | + | <StructureSection load='3fhi' size='900' side='right' caption='Caption for this structure' scene='58/583419/3fhi/1'> |
| - | + | == Structural highlights == | |
| - | + | <scene name='58/583419/Pka_reg/1'>The PKA catalytic unit crystalized with its regulatory unit</scene>, pdb structure [[3fhi]]<ref>PMID:15692043</ref>, is shown with the catalytic unit's small lobe in ''gray'' and large lobe in ''tan'', and the pka regulatory unit in ''light blue''. | |
| - | = | + | The <scene name='58/583419/Pka_reg/4'>residues from exon 1 of the catalytic unit do not directly interact with the regulatory unit</scene>. The residues from exon 1 of the catalytic unit are labelled by residue index. Note that the last three residues from exon 1 (residues # 13-15) are the only amino acids from the first exon of the catalytic unit that were included in this crystal structure. Also note the residues from the catalytic unit's downstream exons, which do in-fact interact with the regulatory unit - these interacting residues are represented with halos and ball-and-stick. As seen in the structure, the exon 1 residues are over 30 angstroms from the regulatory unit - too far to bind. |
| - | = | + | In support of the absence-of-interaction observation, in the crystal structure of the tetromeric holoenzyme, [[3tnp]]<ref>PMID:22323819</ref>, there is also no interaction between <scene name='58/583419/Pka_dimer/3'>exon 1 residues</scene>, shown with halos, to the regulatory units, shown in green and purple. |
| - | + | ---- | |
| - | + | Moreover, the exon 1 residues are also <scene name='58/583419/Pka_reg/3'>distant from PKI-interacting residues (halos and ball and stick)</scene> - a second regulator/inhibitor of the catalytic unit. | |
| - | + | ||
| + | This is also obvious from the pdb structure for the <scene name='58/583419/Pka_pki/1'>crystal of the catalytic unit bound to PKI (purple)</scene>, [[1atp]]<ref>PMID:15299527</ref>. | ||
| + | |||
| + | <!-- | ||
| + | <scene name='58/583419/Hsp40/1'>TextToBeDisplayed</scene>[[1hdj]]<ref>PMID: 8764402</ref> | ||
| + | <scene name='58/583419/Hsp40/2'>TextToBeDisplayed</scene><ref>PMID: 19519518</ref> | ||
| + | --> | ||
</StructureSection> | </StructureSection> | ||
== References == | == References == | ||
<references/> | <references/> | ||
Current revision
PKA and Regulators
| |||||||||||
References
- ↑ Kim C, Xuong NH, Taylor SS. Crystal structure of a complex between the catalytic and regulatory (RIalpha) subunits of PKA. Science. 2005 Feb 4;307(5710):690-6. PMID:15692043 doi:307/5710/690
- ↑ Zhang P, Smith-Nguyen EV, Keshwani MM, Deal MS, Kornev AP, Taylor SS. Structure and allostery of the PKA RIIbeta tetrameric holoenzyme. Science. 2012 Feb 10;335(6069):712-6. PMID:22323819 doi:http://dx.doi.org/10.1126/science.1213979
- ↑ Zheng J, Trafny EA, Knighton DR, Xuong NH, Taylor SS, Ten Eyck LF, Sowadski JM. 2.2 A refined crystal structure of the catalytic subunit of cAMP-dependent protein kinase complexed with MnATP and a peptide inhibitor. Acta Crystallogr D Biol Crystallogr. 1993 May 1;49(Pt 3):362-5. PMID:15299527 doi:10.1107/S0907444993000423
