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4d0o
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 4d0o is ON HOLD Authors: Abdul Azeez, K.R., Shrestha, L., Krojer, T., Allerston, C., von Delft, F., Bountra, C., Arrowsmith, C., Edwards, A.M., Knap...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==AKAP13 (AKAP-Lbc) DH domain== | |
| + | <StructureSection load='4d0o' size='340' side='right'caption='[[4d0o]], [[Resolution|resolution]] 2.75Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[4d0o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4D0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4D0O FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4d0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4d0o OCA], [https://pdbe.org/4d0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4d0o RCSB], [https://www.ebi.ac.uk/pdbsum/4d0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4d0o ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/AKP13_HUMAN AKP13_HUMAN] Anchors cAMP-dependent protein kinase (PKA) and acts as an adapter protein to selectively couple G alpha-13 and Rho. Augments gene activation by the estrogen receptor in an element-specific and ligand-dependent manner. Activates estrogen receptor beta by a p38 MAPK-dependent pathway. Stimulates exchange activity on Rho proteins in vitro, but not on CDC42, Ras or Rac and may bind calcium ions.<ref>PMID:11546812</ref> <ref>PMID:9627117</ref> <ref>PMID:9891067</ref> <ref>PMID:11579095</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The RhoGEF domain of AKAP-Lbc (AKAP13) catalyses nucleotide exchange on RhoA and is involved in development of cardiac hypertrophy. The RhoGEF activity of AKAP-Lbc has also been implicated in cancer. We have determined the X-ray crystal structure of the complex between RhoA:GDP and the AKAP-Lbc RhoGEF (DH-PH) domain to 2.1 A resolution. The structure reveals important differences compared to related RhoGEF proteins such as Leukemia-associated RhoGEF. Nucleotide exchange assays comparing the activity of the DH-PH domain to the DH domain alone showed no role for the PH domain in nucleotide exchange, which is explained by the RhoA:AKAP-Lbc structure. Comparison to a structure of the isolated AKAP-Lbc DH domain revealed a change in conformation of the N-terminal 'GEF switch' region upon binding to RhoA. Isothermal titration calorimetry showed that AKAP-Lbc has only micromolar affinity for RhoA which combined with the presence of potential binding pockets for small molecules on AKAP-Lbc raises the possibility of targeting AKAP-Lbc with guanine nucleotide exchange factor inhibitors. | ||
| - | + | The Crystal Structure of the RhoA : AKAP-Lbc DH-PH Domain Complex.,Abdul Azeez KR, Knapp S, Fernandes JM, Klussmann E, Elkins JM Biochem J. 2014 Sep 4. PMID:25186459<ref>PMID:25186459</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| + | </div> | ||
| + | <div class="pdbe-citations 4d0o" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[A-kinase anchor protein 3D structures|A-kinase anchor protein 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Abdul Azeez KR]] | ||
| + | [[Category: Allerston C]] | ||
| + | [[Category: Arrowsmith C]] | ||
| + | [[Category: Bountra C]] | ||
| + | [[Category: Edwards AM]] | ||
| + | [[Category: Elkins JM]] | ||
| + | [[Category: Klussmann E]] | ||
| + | [[Category: Knapp S]] | ||
| + | [[Category: Krojer T]] | ||
| + | [[Category: Shrestha L]] | ||
| + | [[Category: Von Delft F]] | ||
Current revision
AKAP13 (AKAP-Lbc) DH domain
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Categories: Homo sapiens | Large Structures | Abdul Azeez KR | Allerston C | Arrowsmith C | Bountra C | Edwards AM | Elkins JM | Klussmann E | Knapp S | Krojer T | Shrestha L | Von Delft F
