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1sgt

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[[Image:1sgt.gif|left|200px]]<br /><applet load="1sgt" size="350" color="white" frame="true" align="right" caption="1sgt, resolution 1.7&Aring;" />
 
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'''REFINED CRYSTAL STRUCTURE OF STREPTOMYCES GRISEUS TRYPSIN AT 1.7 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==REFINED CRYSTAL STRUCTURE OF STREPTOMYCES GRISEUS TRYPSIN AT 1.7 ANGSTROMS RESOLUTION==
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Streptomyces griseus trypsin (SGT) is a bacterial serine proteinase that is more homologous to mammalian than to other bacterial enzymes. The structure of SGT has been solved primarily by molecular replacement, though some low-resolution phase information was supplied by heavy-atom derivatives. The mammalian pancreatic serine proteinases bovine trypsin (BT) and alpha-chymotrypsin (CHT) were used as molecular replacement models. Because these proteins have low homology with SGT compared to the majority of other successful replacement models, new strategies were required for molecular replacement to succeed. The model of SGT has been refined at 1.7 A resolution to a final R-factor of 0.161 (1 A = 0.1 nm); the correlation coefficient between all observed and calculated structure factor amplitudes is 0.908. Solvent molecules located in the crystal structure play an important role in stabilizing buried charged and polar groups. An additional contribution to stability can be seen in the fact that the majority of the charged side-chains are involved in ionic interactions, sometimes linking the two domains of SGT. A comparison of SGT with BT shows that the greatest similarities are in the active-site and substrate-binding regions, consistent with their similar substrate specificities. The modeling of complexes of SGT with two inhibitors of BT, pancreatic trypsin inhibitor (PTI) and the third domain of Japanese quail ovomucoid (OMJPQ3), helps to explain why PTI inhibits SGT but OMJPQ3 does not. Like BT, but unlike other bacterial serine proteinases of known structure, SGT has a buried N terminus. SGT has also a well-defined Ca2+-binding site, but this site differs in location from that of BT.
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<StructureSection load='1sgt' size='340' side='right'caption='[[1sgt]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sgt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SGT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SGT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sgt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sgt OCA], [https://pdbe.org/1sgt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sgt RCSB], [https://www.ebi.ac.uk/pdbsum/1sgt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sgt ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/TRYP_STRGR TRYP_STRGR]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sg/1sgt_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sgt ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SGT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_chryseus Streptomyces chryseus] with <scene name='pdbligand=CA:'>CA</scene> as [[ligand]]. Active as [http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SGT OCA].
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*[[Trypsin 3D structures|Trypsin 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Refined crystal structure of Streptomyces griseus trypsin at 1.7 A resolution., Read RJ, James MN, J Mol Biol. 1988 Apr 5;200(3):523-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3135412 3135412]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Streptomyces griseus]]
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[[Category: Streptomyces chryseus]]
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[[Category: James MNG]]
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[[Category: Trypsin]]
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[[Category: Read RJ]]
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[[Category: James, M N.G.]]
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[[Category: Read, R J.]]
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[[Category: CA]]
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[[Category: hydrolase (serine proteinase)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Mar 18 13:26:09 2008''
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Current revision

REFINED CRYSTAL STRUCTURE OF STREPTOMYCES GRISEUS TRYPSIN AT 1.7 ANGSTROMS RESOLUTION

PDB ID 1sgt

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