2wlq

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==NUCLEOPHILE-DISABLED LAM16A MUTANT HOLDS LAMINARIHEPTAOSE (L7) IN A CYCLICAL CONFORMATION==
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<StructureSection load='2wlq' size='340' side='right' caption='[[2wlq]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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==Nucleophile-disabled Lam16A mutant holds laminariheptaose (L7) in a cyclical conformation==
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<StructureSection load='2wlq' size='340' side='right'caption='[[2wlq]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2wlq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Phanerochaete_chrysosporium Phanerochaete chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WLQ OCA]. <br>
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<table><tr><td colspan='2'>[[2wlq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phanerodontia_chrysosporium Phanerodontia chrysosporium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WLQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WLQ FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=ASO:1,5-ANHYDROSORBITOL'>ASO</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2w39|2w39]], [[2w52|2w52]], [[2cl2|2cl2]], [[2wne|2wne]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wlq OCA], [https://pdbe.org/2wlq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wlq RCSB], [https://www.ebi.ac.uk/pdbsum/2wlq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wlq ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2wlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wlq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2wlq RCSB], [http://www.ebi.ac.uk/pdbsum/2wlq PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q874E3_PHACH Q874E3_PHACH]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
<jmolCheckbox>
<jmolCheckbox>
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<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wl/2wlq_consurf.spt"</scriptWhenChecked>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wl/2wlq_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
<text>to colour the structure by Evolutionary Conservation</text>
<text>to colour the structure by Evolutionary Conservation</text>
</jmolCheckbox>
</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wlq ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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Synthesis of Cyclic beta-Glucan Using Laminarinase 16A Glycosynthase Mutant from the Basidiomycete Phanerochaete chrysosporium.,Vasur J, Kawai R, Jonsson KH, Widmalm G, Engstrom A, Frank M, Andersson E, Hansson H, Forsberg Z, Igarashi K, Samejima M, Sandgren M, Stahlberg J J Am Chem Soc. 2010 Jan 15. PMID:20078120<ref>PMID:20078120</ref>
Synthesis of Cyclic beta-Glucan Using Laminarinase 16A Glycosynthase Mutant from the Basidiomycete Phanerochaete chrysosporium.,Vasur J, Kawai R, Jonsson KH, Widmalm G, Engstrom A, Frank M, Andersson E, Hansson H, Forsberg Z, Igarashi K, Samejima M, Sandgren M, Stahlberg J J Am Chem Soc. 2010 Jan 15. PMID:20078120<ref>PMID:20078120</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
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<div class="pdbe-citations 2wlq" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Phanerochaete chrysosporium]]
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[[Category: Large Structures]]
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[[Category: Andersson, E.]]
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[[Category: Phanerodontia chrysosporium]]
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[[Category: Engstrom, A.]]
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[[Category: Andersson E]]
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[[Category: Hansson, H.]]
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[[Category: Engstrom A]]
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[[Category: Igarashi, K.]]
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[[Category: Hansson H]]
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[[Category: Jonsson, K H.]]
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[[Category: Igarashi K]]
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[[Category: Kawai, R.]]
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[[Category: Jonsson KH]]
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[[Category: Samejima, M.]]
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[[Category: Kawai R]]
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[[Category: Sandgren, M.]]
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[[Category: Samejima M]]
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[[Category: Stahlberg, J.]]
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[[Category: Sandgren M]]
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[[Category: Vasur, J.]]
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[[Category: Stahlberg J]]
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[[Category: Widmalm, G.]]
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[[Category: Vasur J]]
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[[Category: 6-glucan]]
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[[Category: Widmalm G]]
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[[Category: Basidiomycete]]
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[[Category: Beta sandwich]]
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[[Category: Beta-1\]]
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[[Category: Beta-glucanase]]
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[[Category: Cyclical poysaccharide]]
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[[Category: Family 16]]
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[[Category: Gh16]]
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[[Category: Gh7]]
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[[Category: Glycosyl hydrolase]]
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[[Category: Hydrolase]]
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[[Category: Lam16a]]
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[[Category: Laminarin]]
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Current revision

Nucleophile-disabled Lam16A mutant holds laminariheptaose (L7) in a cyclical conformation

PDB ID 2wlq

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