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| | ==Solution structure of the Get5 carboxyl domain from S. cerevisiae== | | ==Solution structure of the Get5 carboxyl domain from S. cerevisiae== |
| - | <StructureSection load='2lnz' size='340' side='right' caption='[[2lnz]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2lnz' size='340' side='right'caption='[[2lnz]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lnz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNZ OCA]. <br> | + | <table><tr><td colspan='2'>[[2lnz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LNZ FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3vej|3vej]], [[2lo0|2lo0]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GET5, MDY2, TMA24, YOL111C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnz OCA], [https://pdbe.org/2lnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lnz RCSB], [https://www.ebi.ac.uk/pdbsum/2lnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnz ProSAT]</span></td></tr> |
| - | <tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr> | + | </table> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lnz RCSB], [http://www.ebi.ac.uk/pdbsum/2lnz PDBsum]</span></td></tr>
| + | == Function == |
| - | <table> | + | [https://www.uniprot.org/uniprot/MDY2_YEAST MDY2_YEAST] Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.<ref>PMID:10514570</ref> <ref>PMID:16390866</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | Get5 Carboxyl-terminal Domain Is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex.,Chartron JW, Vandervelde DG, Rao M, Clemons WM Jr J Biol Chem. 2012 Mar 9;287(11):8310-7. Epub 2012 Jan 17. PMID:22262836<ref>PMID:22262836</ref> | | Get5 Carboxyl-terminal Domain Is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex.,Chartron JW, Vandervelde DG, Rao M, Clemons WM Jr J Biol Chem. 2012 Mar 9;287(11):8310-7. Epub 2012 Jan 17. PMID:22262836<ref>PMID:22262836</ref> |
| | | | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 2lnz" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Saccharomyces cerevisiae]] | + | [[Category: Large Structures]] |
| - | [[Category: Chartron, J W.]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Clemons, W M.]] | + | [[Category: Chartron JW]] |
| - | [[Category: Rao, M.]] | + | [[Category: Clemons Jr WM]] |
| - | [[Category: Vandervelde, D G.]] | + | [[Category: Rao M]] |
| - | [[Category: Dimerization]]
| + | [[Category: Vandervelde DG]] |
| - | [[Category: Homodimerization]]
| + | |
| - | [[Category: Protein binding]]
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| Structural highlights
Function
MDY2_YEAST Required for efficient mating. Involved in the production of alpha-factor, the KAR9 and TUB1 location to the shmoo tip and nuclear migration into pheromone-induced shmoos.[1] [2]
Publication Abstract from PubMed
Tail-anchored trans-membrane proteins are targeted to membranes post-translationally. The proteins Get4 and Get5 form an obligate complex that catalyzes the transfer of tail-anchored proteins destined to the endoplasmic reticulum from Sgt2 to the cytosolic targeting factor Get3. Get5 forms a homodimer mediated by its carboxyl domain. We show here that a conserved motif exists within the carboxyl domain. A high resolution crystal structure and solution NMR structures of this motif reveal a novel and stable helical dimerization domain. We additionally determined a solution NMR structure of a divergent fungal homolog, and comparison of these structures allows annotation of specific stabilizing interactions. Using solution x-ray scattering and the structures of all folded domains, we present a model of the full-length Get4/Get5 complex.
Get5 Carboxyl-terminal Domain Is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex.,Chartron JW, Vandervelde DG, Rao M, Clemons WM Jr J Biol Chem. 2012 Mar 9;287(11):8310-7. Epub 2012 Jan 17. PMID:22262836[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Iwanejko L, Smith KN, Loeillet S, Nicolas A, Fabre F. Disruption and functional analysis of six ORFs on chromosome XV: YOL117w, YOL115w ( TRF4), YOL114c, YOL112w ( MSB4), YOL111c and YOL072w. Yeast. 1999 Oct;15(14):1529-39. PMID:10514570 doi:<1529::AID-YEA457>3.0.CO;2-Y 10.1002/(SICI)1097-0061(199910)15:14<1529::AID-YEA457>3.0.CO;2-Y
- ↑ Hu Z, Potthoff B, Hollenberg CP, Ramezani-Rad M. Mdy2, a ubiquitin-like (UBL)-domain protein, is required for efficient mating in Saccharomyces cerevisiae. J Cell Sci. 2006 Jan 15;119(Pt 2):326-38. Epub 2006 Jan 3. PMID:16390866 doi:jcs.02754
- ↑ Chartron JW, Vandervelde DG, Rao M, Clemons WM Jr. Get5 Carboxyl-terminal Domain Is a Novel Dimerization Motif That Tethers an Extended Get4/Get5 Complex. J Biol Chem. 2012 Mar 9;287(11):8310-7. Epub 2012 Jan 17. PMID:22262836 doi:10.1074/jbc.M111.333252
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