2mox

Current revision

solution structure of tandem SH3 domain of Sorbin and SH3 domain-containing protein 1

Structural highlights

2mox is a 1 chain structure with sequence from Homo sapiens. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SRBS1_HUMAN Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions (By similarity).[UniProtKB:Q62417]

Publication Abstract from PubMed

c-Cbl-associated protein (CAP) is an important cytoskeletal adaptor protein involved in the regulation of adhesion turnover. The interaction between CAP and vinculin is critical for the recruitment of CAP to focal adhesions. The tandem SH3 domains (herein termed SH3a and SH3b) of CAP are responsible for its interaction with vinculin. However, the structural mechanism underlying the interaction between CAP and vinculin is poorly understood. In this manuscript, we report the solution structure of the tandem SH3 domains of CAP. Our NMR and ITC data indicate that the SH3a and SH3b domains of CAP simultaneously bind to a long proline-rich region of vinculin with different binding specificities. Furthermore, the crystal structures of the individual SH3a and SH3b domains complexed with their substrate peptides indicate that Q807(SH3a) and D881(SH3b) are the critical residues determining the different binding specificities of the SH3 domains. Based on the obtained structural information, a model of the SH3ab-vinculin complex was generated using MD simulation and SAXS data.

Structural investigation of the interaction between the tandem SH3 domains of c-Cbl-associated protein and vinculin.,Zhao D, Wang X, Peng J, Wang C, Li F, Sun Q, Zhang Y, Zhang J, Cai G, Zuo X, Wu J, Shi Y, Zhang Z, Gong Q J Struct Biol. 2014 Aug;187(2):194-205. doi: 10.1016/j.jsb.2014.05.009. Epub 2014, May 28. PMID:24878663^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Zhao D, Wang X, Peng J, Wang C, Li F, Sun Q, Zhang Y, Zhang J, Cai G, Zuo X, Wu J, Shi Y, Zhang Z, Gong Q. Structural investigation of the interaction between the tandem SH3 domains of c-Cbl-associated protein and vinculin. J Struct Biol. 2014 Aug;187(2):194-205. doi: 10.1016/j.jsb.2014.05.009. Epub 2014, May 28. PMID:24878663 doi:http://dx.doi.org/10.1016/j.jsb.2014.05.009

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2mox"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 2mox is ON HOLD
+==solution structure of tandem SH3 domain of Sorbin and SH3 domain-containing protein 1==
+<StructureSection load='2mox' size='340' side='right'caption='[[2mox]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2mox]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MOX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MOX FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mox FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mox OCA], [https://pdbe.org/2mox PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mox RCSB], [https://www.ebi.ac.uk/pdbsum/2mox PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mox ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/SRBS1_HUMAN SRBS1_HUMAN] Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions (By similarity).[UniProtKB:Q62417]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+c-Cbl-associated protein (CAP) is an important cytoskeletal adaptor protein involved in the regulation of adhesion turnover. The interaction between CAP and vinculin is critical for the recruitment of CAP to focal adhesions. The tandem SH3 domains (herein termed SH3a and SH3b) of CAP are responsible for its interaction with vinculin. However, the structural mechanism underlying the interaction between CAP and vinculin is poorly understood. In this manuscript, we report the solution structure of the tandem SH3 domains of CAP. Our NMR and ITC data indicate that the SH3a and SH3b domains of CAP simultaneously bind to a long proline-rich region of vinculin with different binding specificities. Furthermore, the crystal structures of the individual SH3a and SH3b domains complexed with their substrate peptides indicate that Q807(SH3a) and D881(SH3b) are the critical residues determining the different binding specificities of the SH3 domains. Based on the obtained structural information, a model of the SH3ab-vinculin complex was generated using MD simulation and SAXS data.
-Authors: Zhao, D., Wang, C., Zhang, J., Wu, J., Shi, Y., Zhang, Z., Gong, Q.
+Structural investigation of the interaction between the tandem SH3 domains of c-Cbl-associated protein and vinculin.,Zhao D, Wang X, Peng J, Wang C, Li F, Sun Q, Zhang Y, Zhang J, Cai G, Zuo X, Wu J, Shi Y, Zhang Z, Gong Q J Struct Biol. 2014 Aug;187(2):194-205. doi: 10.1016/j.jsb.2014.05.009. Epub 2014, May 28. PMID:24878663<ref>PMID:24878663</ref>
-Description: solution structure of tandem SH3 domain of Sorbin and SH3 domain-containing protein 1
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2mox" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Gong Q]]
+[[Category: Shi Y]]
+[[Category: Wang C]]
+[[Category: Wu J]]
+[[Category: Zhang J]]
+[[Category: Zhang Z]]
+[[Category: Zhao D]]