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2yfs

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==CRYSTAL STRUCTURE OF INULOSUCRASE FROM LACTOBACILLUS JOHNSONII NCC533 IN COMPLEX WITH SUCROSE==
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<StructureSection load='2yfs' size='340' side='right' caption='[[2yfs]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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==Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with sucrose==
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<StructureSection load='2yfs' size='340' side='right'caption='[[2yfs]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2yfs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_johnsonii Lactobacillus johnsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFS OCA]. <br>
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<table><tr><td colspan='2'>[[2yfs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactobacillus_johnsonii Lactobacillus johnsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2YFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2YFS FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2yft|2yft]], [[2yfr|2yfr]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=FRU:FRUCTOSE'>FRU</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900003:sucrose'>PRD_900003</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2yfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfs OCA], [https://pdbe.org/2yfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2yfs RCSB], [https://www.ebi.ac.uk/pdbsum/2yfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2yfs ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2yfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2yfs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2yfs RCSB], [http://www.ebi.ac.uk/pdbsum/2yfs PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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[https://www.uniprot.org/uniprot/INUS_LACJO INUS_LACJO] Fructosyltransferase that catalyzes the polymerization of the fructose moiety of sucrose to produce inulin polymer and inulin oligosaccharides such as 1-kestose and nystose.<ref>PMID:18408060</ref> <ref>PMID:21801732</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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Crystal Structure of Inulosucrase from Lactobacillus: Insights into the Substrate Specificity and Product Specificity of GH68 Fructansucrases.,Pijning T, Anwar MA, Boger M, Dobruchowska JM, Leemhuis H, Kralj S, Dijkhuizen L, Dijkstra BW J Mol Biol. 2011 Sep 9;412(1):80-93. Epub 2011 Jul 23. PMID:21801732<ref>PMID:21801732</ref>
Crystal Structure of Inulosucrase from Lactobacillus: Insights into the Substrate Specificity and Product Specificity of GH68 Fructansucrases.,Pijning T, Anwar MA, Boger M, Dobruchowska JM, Leemhuis H, Kralj S, Dijkhuizen L, Dijkstra BW J Mol Biol. 2011 Sep 9;412(1):80-93. Epub 2011 Jul 23. PMID:21801732<ref>PMID:21801732</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
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<div class="pdbe-citations 2yfs" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Inulosucrase]]
 
[[Category: Lactobacillus johnsonii]]
[[Category: Lactobacillus johnsonii]]
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[[Category: Anwar, M A.]]
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[[Category: Large Structures]]
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[[Category: Dijkhuizen, L.]]
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[[Category: Anwar MA]]
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[[Category: Dijkstra, B W.]]
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[[Category: Dijkhuizen L]]
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[[Category: Kralj, S.]]
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[[Category: Dijkstra BW]]
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[[Category: Leemhuis, H.]]
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[[Category: Kralj S]]
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[[Category: Pijning, T.]]
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[[Category: Leemhuis H]]
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[[Category: Fructosyltransferase]]
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[[Category: Pijning T]]
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[[Category: Glycoside hydrolase family gh68]]
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[[Category: Sugar utilization]]
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[[Category: Transferase]]
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Current revision

Crystal structure of inulosucrase from Lactobacillus johnsonii NCC533 in complex with sucrose

PDB ID 2yfs

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