2wyf
From Proteopedia
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| - | == | + | |
| - | <StructureSection load='2wyf' size='340' side='right' caption='[[2wyf]], [[Resolution|resolution]] 2.40Å' scene=''> | + | ==Crystal structure of PA-IL lectin complexed with aGal12bGal-O-Met at 2.4 A resolution== |
| + | <StructureSection load='2wyf' size='340' side='right'caption='[[2wyf]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[2wyf]] is a 8 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[2wyf]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2WYF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2WYF FirstGlance]. <br> |
| - | </ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene>, <scene name='pdbligand=MBG:METHYL-BETA-GALACTOSE'>MBG</scene></td></tr> | |
| - | <tr><td class="sblockLbl"><b> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2wyf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2wyf OCA], [https://pdbe.org/2wyf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2wyf RCSB], [https://www.ebi.ac.uk/pdbsum/2wyf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2wyf ProSAT]</span></td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </table> |
| - | <table> | + | == Function == |
| + | [https://www.uniprot.org/uniprot/PA1L_PSEAE PA1L_PSEAE] D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wy/2wyf_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wy/2wyf_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2wyf ConSurf]. |
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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Role of water molecules in structure and energetics of Pseudomonas aeruginosa lectin I interacting with disaccharides.,Nurisso A, Blanchard B, Audfray A, Rydner L, Oscarson S, Varrot A, Imberty A J Biol Chem. 2010 Jun 25;285(26):20316-27. Epub 2010 Apr 21. PMID:20410292<ref>PMID:20410292</ref> | Role of water molecules in structure and energetics of Pseudomonas aeruginosa lectin I interacting with disaccharides.,Nurisso A, Blanchard B, Audfray A, Rydner L, Oscarson S, Varrot A, Imberty A J Biol Chem. 2010 Jun 25;285(26):20316-27. Epub 2010 Apr 21. PMID:20410292<ref>PMID:20410292</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
</div> | </div> | ||
| + | <div class="pdbe-citations 2wyf" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Galactose-binding lectin|Galactose-binding lectin]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: Pseudomonas aeruginosa]] | + | [[Category: Large Structures]] |
| - | [[Category: Blanchard | + | [[Category: Pseudomonas aeruginosa PAO1]] |
| - | [[Category: Imberty | + | [[Category: Blanchard B]] |
| - | [[Category: Nurisso | + | [[Category: Imberty A]] |
| - | [[Category: Varrot | + | [[Category: Nurisso A]] |
| - | + | [[Category: Varrot A]] | |
| - | + | ||
Current revision
Crystal structure of PA-IL lectin complexed with aGal12bGal-O-Met at 2.4 A resolution
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