183d

[[Image:183d.gif|left|200px]]

|PDB= 183d |SIZE=350|CAPTION= <scene name='initialview01'>183d</scene>, resolution 1.600&Aring;

 

'''X-RAY STRUCTURE OF A DNA DECAMER CONTAINING 7, 8-DIHYDRO-8-OXOGUANINE'''

 

 

==Overview==

We have determined the x-ray structure of a DNA fragment containing 7,8-dihydro-8-oxoguanine (G(O)). The structure of the duplex form of d(CCAGOCGCTGG) has been determined to 1.6-A resolution. The results demonstrate that GO forms Watson-Crick base pairs with the opposite C and that G(O) is in the anti conformation. Structural perturbations induced by C.G(O)anti base pairs are subtle. The structure allows us to identify probable elements by which the DNA repair protein MutM recognizes its substrates. Hydrogen bond donors/acceptors within the major groove are the most likely element. In that groove, the pattern of hydrogen-bond donors/acceptors of C.G(O)anti is unique. Additional structural analysis indicates that conversion of G to G(O) would not significantly influence the glycosidic torsion preference of the nucleoside. There is no steric interaction of the 8-oxygen of G(O) with the phospho-deoxyribose backbone.

 

==About this Structure==

183D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=183D OCA].

 

==Reference==

X-ray structure of a DNA decamer containing 7,8-dihydro-8-oxoguanine., Lipscomb LA, Peek ME, Morningstar ML, Verghis SM, Miller EM, Rich A, Essigmann JM, Williams LD, Proc Natl Acad Sci U S A. 1995 Jan 31;92(3):719-23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7846041 7846041]

[[Category: Protein complex]]

[[Category: Essigmann, J M.]]

[[Category: Lipscomb, L A.]]

[[Category: Miller, E M.]]

[[Category: Morningstar, M L.]]

[[Category: Peek, M E.]]

[[Category: Rich, A.]]

[[Category: Verghis, S M.]]

[[Category: Williams, L D.]]