|
|
| (3 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | + | |
| | ==Structure of the psedudokinase domain of BIR2, an immune regulator of the RLK/Pelle family== | | ==Structure of the psedudokinase domain of BIR2, an immune regulator of the RLK/Pelle family== |
| - | <StructureSection load='4l68' size='340' side='right' caption='[[4l68]], [[Resolution|resolution]] 2.00Å' scene=''> | + | <StructureSection load='4l68' size='340' side='right'caption='[[4l68]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4l68]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4L68 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4l68]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4L68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4L68 FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PDO:1,3-PROPANDIOL'>PDO</scene><br> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AT3G28450, At3g28450/MFJ20_13, LRR-RLK ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PDO:1,3-PROPANDIOL'>PDO</scene></td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4l68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l68 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4l68 RCSB], [http://www.ebi.ac.uk/pdbsum/4l68 PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4l68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4l68 OCA], [https://pdbe.org/4l68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4l68 RCSB], [https://www.ebi.ac.uk/pdbsum/4l68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4l68 ProSAT]</span></td></tr> |
| - | <table> | + | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/BIR2_ARATH BIR2_ARATH] Pseudokinases lacking protein kinase activity and unable to bind ATP-analogs (PubMed:24556575). Negative regulator of pathogen-associated molecular patterns- (PAMP-) triggered immunity by limiting BAK1-receptor complex formation in the absence of ligands (PubMed:24388849).<ref>PMID:24388849</ref> <ref>PMID:24556575</ref> |
| - | The BAK1-interacting receptor-like kinase 2 (BIR2) belongs to the large family of leucine-rich repeat receptor-like kinases (LRR-RLKs) that mediate development and innate immunity in plants and form a monophyletic gene family with the Drosophila Pelle and human interleukin-1 receptor-associated kinases (IRAK). BIR2 is a negative regulator of BAK1-mediated defense mechanisms and cell death responses, yet key residues that are typically required for kinase activity are not present in the BIR2 kinase domain. We have determined the crystal structure of the BIR2 cytosolic domain and show that its nucleotide binding site is occluded. NMR spectroscopy confirmed that neither wild type nor phosphorylation-mimicking mutants of BIR2 bind ATP-analogues in solution, suggesting that BIR2 is a genuine enzymatically inactive pseudokinase. BIR2 is, however, phosphorylated by its target of regulation, BAK1. Using nano LC-MS/MS analysis for site-specific analysis of phosphorylation, we found a high density of BAK1-transphosphorylation sites in the BIR2 juxta membrane domain, a region previously implicated in regulation of RLKs. Our findings provide a structural basis to better understand signaling through kinase-dead domains that are predicted to account for 20% of all Arabidopsis RLKs and 10% of all human kinases.
| + | |
| - | | + | |
| - | Structure of the pseudokinase domain of BIR2, a regulator of BAK1-mediated immune signaling in Arabidopsis.,Blaum BS, Mazzotta S, Noldeke ER, Halter T, Madlung J, Kemmerling B, Stehle T J Struct Biol. 2014 Apr;186(1):112-21. doi: 10.1016/j.jsb.2014.02.005. Epub 2014 , Feb 17. PMID:24556575<ref>PMID:24556575</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div> | + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| - | [[Category: Blaum, B S.]] | + | [[Category: Large Structures]] |
| - | [[Category: Stehle, T.]] | + | [[Category: Blaum BS]] |
| - | [[Category: Negative immune regulator]] | + | [[Category: Stehle T]] |
| - | [[Category: Pseudokinase]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
BIR2_ARATH Pseudokinases lacking protein kinase activity and unable to bind ATP-analogs (PubMed:24556575). Negative regulator of pathogen-associated molecular patterns- (PAMP-) triggered immunity by limiting BAK1-receptor complex formation in the absence of ligands (PubMed:24388849).[1] [2]
References
- ↑ Halter T, Imkampe J, Mazzotta S, Wierzba M, Postel S, Bucherl C, Kiefer C, Stahl M, Chinchilla D, Wang X, Nurnberger T, Zipfel C, Clouse S, Borst JW, Boeren S, de Vries SC, Tax F, Kemmerling B. The leucine-rich repeat receptor kinase BIR2 is a negative regulator of BAK1 in plant immunity. Curr Biol. 2014 Jan 20;24(2):134-43. doi: 10.1016/j.cub.2013.11.047. Epub 2014, Jan 2. PMID:24388849 doi:http://dx.doi.org/10.1016/j.cub.2013.11.047
- ↑ Blaum BS, Mazzotta S, Noldeke ER, Halter T, Madlung J, Kemmerling B, Stehle T. Structure of the pseudokinase domain of BIR2, a regulator of BAK1-mediated immune signaling in Arabidopsis. J Struct Biol. 2014 Apr;186(1):112-21. doi: 10.1016/j.jsb.2014.02.005. Epub 2014 , Feb 17. PMID:24556575 doi:http://dx.doi.org/10.1016/j.jsb.2014.02.005
|
