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1a5q

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[[Image:1a5q.gif|left|200px]]
 
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{{Structure
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==P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A==
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|PDB= 1a5q |SIZE=350|CAPTION= <scene name='initialview01'>1a5q</scene>, resolution 2.3&Aring;
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<StructureSection load='1a5q' size='340' side='right'caption='[[1a5q]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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<table><tr><td colspan='2'>[[1a5q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A5Q FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a5q OCA], [https://pdbe.org/1a5q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a5q RCSB], [https://www.ebi.ac.uk/pdbsum/1a5q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a5q ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a5/1a5q_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a5q ConSurf].
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<div style="clear:both"></div>
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'''P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A'''
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==See Also==
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Overview==
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<references/>
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The Tyr92-Pro93 peptide group of bovine pancreatic ribonuclease A (RNase A) exists in the cis conformation in the native state. From unfolding/refolding kinetic studies of the disulfide-intact wild-type protein and of a variant in which Pro93 had been replaced by Ala, it had been suggested that the Tyr92-Ala93 peptide group also exists in the cis conformation in the native state. Here, we report the crystal structure of the P93A variant. Although there is disorder in the region of residues 92 and 93, the best structural model contains a cis peptide at this position, lending support to the results of the kinetics experiments. We also report the crystal structure of the C[40, 95]A variant, which is an analog of the major rate-determining three-disulfide intermediate in the oxidative folding of RNase A, missing the 40-95 disulfide bond. As had been detected by NMR spectroscopy, the crystal structure of this analog shows disorder in the region surrounding the missing disulfide. However, the global chain fold of the remainder of the protein, including the disulfide bond between Cys65 and Cys72, appears to be unaffected by the mutation.
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__TOC__
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</StructureSection>
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==About this Structure==
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1A5Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A5Q OCA].
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==Reference==
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Crystal structures of two mutants that have implications for the folding of bovine pancreatic ribonuclease A., Pearson MA, Karplus PA, Dodge RW, Laity JH, Scheraga HA, Protein Sci. 1998 May;7(5):1255-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9605332 9605332]
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[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Dodge RW]]
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[[Category: Dodge, R W.]]
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[[Category: Karplus PA]]
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[[Category: Karplus, P A.]]
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[[Category: Laity JH]]
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[[Category: Laity, J H.]]
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[[Category: Pearson MA]]
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[[Category: Pearson, M A.]]
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[[Category: Scheraga HA]]
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[[Category: Scheraga, H A.]]
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[[Category: SO4]]
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[[Category: hydrolase (nucleic acid]]
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[[Category: rna)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:53:16 2008''
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Current revision

P93A VARIANT OF BOVINE PANCREATIC RIBONUCLEASE A

PDB ID 1a5q

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