4fzp

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==Crystal Structure of the uranyl binding protein complexed with uranyl==
==Crystal Structure of the uranyl binding protein complexed with uranyl==
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<StructureSection load='4fzp' size='340' side='right' caption='[[4fzp]], [[Resolution|resolution]] 1.29&Aring;' scene=''>
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<StructureSection load='4fzp' size='340' side='right'caption='[[4fzp]], [[Resolution|resolution]] 1.29&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4fzp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Metth Metth]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FZP FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4fzp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._Delta_H Methanothermobacter thermautotrophicus str. Delta H]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FZP FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.29&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4fzo|4fzo]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IUM:URANYL+(VI)+ION'>IUM</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MTH_1690 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=187420 METTH])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzp OCA], [https://pdbe.org/4fzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fzp RCSB], [https://www.ebi.ac.uk/pdbsum/4fzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fzp ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fzp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4fzp RCSB], [http://www.ebi.ac.uk/pdbsum/4fzp PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/O27725_METTH O27725_METTH]
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== Publication Abstract from PubMed ==
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Uranyl (UO2(2+)), the predominant aerobic form of uranium, is present in the ocean at a concentration of ~3.2 parts per 10(9) (13.7 nM); however, the successful enrichment of uranyl from this vast resource has been limited by the high concentrations of metal ions of similar size and charge, which makes it difficult to design a binding motif that is selective for uranyl. Here we report the design and rational development of a uranyl-binding protein using a computational screening process in the initial search for potential uranyl-binding sites. The engineered protein is thermally stable and offers very high affinity and selectivity for uranyl with a Kd of 7.4 femtomolar (fM) and &gt;10,000-fold selectivity over other metal ions. We also demonstrated that the uranyl-binding protein can repeatedly sequester 30-60% of the uranyl in synthetic sea water. The chemical strategy employed here may be applied to engineer other selective metal-binding proteins for biotechnology and remediation applications.
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A protein engineered to bind uranyl selectively and with femtomolar affinity.,Zhou L, Bosscher M, Zhang C, Ozcubukcu S, Zhang L, Zhang W, Li CJ, Liu J, Jensen MP, Lai L, He C Nat Chem. 2014 Mar;6(3):236-41. doi: 10.1038/nchem.1856. Epub 2014 Jan 26. PMID:24557139<ref>PMID:24557139</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Metth]]
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[[Category: Large Structures]]
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[[Category: He, C.]]
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[[Category: Methanothermobacter thermautotrophicus str. Delta H]]
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[[Category: Zhang, L.]]
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[[Category: He C]]
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[[Category: Zhou, L.]]
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[[Category: Zhang L]]
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[[Category: Unknown function]]
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[[Category: Zhou L]]
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[[Category: Uranyl binding]]
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Current revision

Crystal Structure of the uranyl binding protein complexed with uranyl

PDB ID 4fzp

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