4pko

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(New page: '''Unreleased structure''' The entry 4pko is ON HOLD Authors: Fei, X., Ye, X., Laronde-Leblanc, N., Lorimer, G.H. Description: Crystal structure of the Football-shaped GroEL-GroES2-(AD...)
Current revision (08:29, 9 October 2024) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 4pko is ON HOLD
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==Crystal structure of the Football-shaped GroEL-GroES2-(ADPBeFx)14 complex==
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<StructureSection load='4pko' size='340' side='right'caption='[[4pko]], [[Resolution|resolution]] 3.84&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4pko]] is a 28 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PKO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PKO FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.84&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pko FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pko OCA], [https://pdbe.org/4pko PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pko RCSB], [https://www.ebi.ac.uk/pdbsum/4pko PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pko ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q7BGE6_ECOLX Q7BGE6_ECOLX] Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter (By similarity).[SAAS:SAAS018369_004_006304][RuleBase:RU000535][HAMAP-Rule:MF_00580]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The GroE chaperonins assist substrate protein (SP) folding by cycling through several conformational states. With each cycle the SP is, in turn, captured, unfolded, briefly encapsulated (t1/2 approximately 1 s), and released by the chaperonin complex. The protein-folding functional form is the US-football-shaped GroEL:GroES2 complex. We report structures of two such "football" complexes to approximately 3.7-A resolution; one is empty whereas the other contains encapsulated SP in both chambers. Although encapsulated SP is not visible on the electron density map, using calibrated FRET and order-of-addition experiments we show that owing to SP-catalyzed ADP/ATP exchange both chambers of the football complex encapsulate SP efficiently only if the binding of SP precedes that of ATP. The two rings of GroEL thus behave as a parallel processing machine, rather than functioning alternately. Compared with the bullet-shaped GroEL:GroES1 complex, the GroEL:GroES2 football complex differs conformationally at the GroEL-GroES interface and also at the interface between the two GroEL rings. We propose that the electrostatic interactions between the epsilon-NH3+ of K105 of helix D in one ring with the negatively charged carboxyl oxygen of A109 at the carboxyl end of helix D of the other ring provide the structural basis for negative inter-ring cooperativity.
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Authors: Fei, X., Ye, X., Laronde-Leblanc, N., Lorimer, G.H.
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Formation and structures of GroEL:GroES2 chaperonin footballs, the protein-folding functional form.,Fei X, Ye X, LaRonde NA, Lorimer GH Proc Natl Acad Sci U S A. 2014 Aug 18. pii: 201412922. PMID:25136110<ref>PMID:25136110</ref>
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Description: Crystal structure of the Football-shaped GroEL-GroES2-(ADPBeFx)14 complex
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4pko" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Chaperonin 3D structures|Chaperonin 3D structures]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Large Structures]]
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[[Category: Fei X]]
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[[Category: Laronde-Leblanc N]]
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[[Category: Lorimer GH]]
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[[Category: Ye X]]

Current revision

Crystal structure of the Football-shaped GroEL-GroES2-(ADPBeFx)14 complex

PDB ID 4pko

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