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1a99

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[[Image:1a99.gif|left|200px]]
 
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{{Structure
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==PUTRESCINE RECEPTOR (POTF) FROM E. COLI==
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|PDB= 1a99 |SIZE=350|CAPTION= <scene name='initialview01'>1a99</scene>, resolution 2.2&Aring;
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<StructureSection load='1a99' size='340' side='right'caption='[[1a99]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene>
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<table><tr><td colspan='2'>[[1a99]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A99 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1A99 FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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|GENE= PUCPOTF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PUT:1,4-DIAMINOBUTANE'>PUT</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1a99 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1a99 OCA], [https://pdbe.org/1a99 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1a99 RCSB], [https://www.ebi.ac.uk/pdbsum/1a99 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1a99 ProSAT]</span></td></tr>
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</table>
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'''PUTRESCINE RECEPTOR (POTF) FROM E. COLI'''
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== Function ==
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[https://www.uniprot.org/uniprot/POTF_ECOLI POTF_ECOLI] Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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PotF protein is a periplasmic substrate-binding protein of the putrescine transport system in Escherichia coli. We have determined the crystal structure of PotF protein in complex with the substrate at 2.3-A resolution. The PotF molecule has dimensions of 54 x 42 x 30 A and consists of two similar globular domains. The PotF structure is reminiscent of other periplasmic receptors with a highest structural homology to another polyamine-binding protein, PotD. Putrescine is tightly bound in the deep cleft between the two domains of PotF through 12 hydrogen bonds and 36 van der Waals interactions. The comparison of the PotF structure with that of PotD provides the insight into the differences in the specificity between the two proteins. The PotF structure, in combination with the mutational analysis, revealed the residues crucial for putrescine binding (Trp-37, Ser-85, Glu-185, Trp-244, Asp-247, and Asp-278) and the importance of water molecules for putrescine recognition.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/a9/1a99_consurf.spt"</scriptWhenChecked>
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1A99 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1A99 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structure and mutational analysis of the Escherichia coli putrescine receptor. Structural basis for substrate specificity., Vassylyev DG, Tomitori H, Kashiwagi K, Morikawa K, Igarashi K, J Biol Chem. 1998 Jul 10;273(28):17604-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9651355 9651355]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1a99 ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Igarashi, K.]]
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[[Category: Igarashi K]]
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[[Category: Kashiwagi, K.]]
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[[Category: Kashiwagi K]]
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[[Category: Morikawa, K.]]
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[[Category: Morikawa K]]
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[[Category: Tomitori, H.]]
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[[Category: Tomitori H]]
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[[Category: Vassylyev, D G.]]
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[[Category: Vassylyev DG]]
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[[Category: PUT]]
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[[Category: binding protein]]
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[[Category: periplasmic putrescine binding protein (potf)]]
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[[Category: transport]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:54:44 2008''
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Current revision

PUTRESCINE RECEPTOR (POTF) FROM E. COLI

PDB ID 1a99

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