4okk

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==Crystal structure of RNase AS from M tuberculosis in complex with UMP==
==Crystal structure of RNase AS from M tuberculosis in complex with UMP==
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<StructureSection load='4okk' size='340' side='right' caption='[[4okk]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
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<StructureSection load='4okk' size='340' side='right'caption='[[4okk]], [[Resolution|resolution]] 2.21&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4okk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OKK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OKK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4okk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OKK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OKK FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.21&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4oke|4oke]], [[4okj|4okj]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=U5P:URIDINE-5-MONOPHOSPHATE'>U5P</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4okk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4okk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4okk RCSB], [http://www.ebi.ac.uk/pdbsum/4okk PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4okk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4okk OCA], [https://pdbe.org/4okk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4okk RCSB], [https://www.ebi.ac.uk/pdbsum/4okk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4okk ProSAT]</span></td></tr>
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<table>
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</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/EXRBN_MYCTU EXRBN_MYCTU] Exonuclease that cleaves single-stranded 3' overhangs of double-stranded RNA. Has no activity with 5' overhangs. Has negligible endonuclease activity. Can bind ATP, dATP and AMP (in vitro); the nucleotide occupies the predicted substrate binding site.<ref>PMID:24311791</ref>
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The cell-envelope of Mycobacterium tuberculosis plays a key role in bacterial virulence and antibiotic resistance. Little is known about the molecular mechanisms of regulation of cell-envelope formation. Here, we elucidate functional and structural properties of RNase AS, which modulates M. tuberculosis cell-envelope properties and strongly impacts bacterial virulence in vivo. The structure of RNase AS reveals a resemblance to RNase T from Escherichia coli, an RNase of the DEDD family involved in RNA maturation. We show that RNase AS acts as a 3'-5'-exoribonuclease that specifically hydrolyzes adenylate-containing RNA sequences. Also, crystal structures of complexes with AMP and UMP reveal the structural basis for the observed enzyme specificity. Notably, RNase AS shows a mechanism of substrate recruitment, based on the recognition of the hydrogen bond donor NH2 group of adenine. Our work opens a field for the design of drugs able to reduce bacterial virulence in vivo.
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Structure and Function of RNase AS, a Polyadenylate-Specific Exoribonuclease Affecting Mycobacterial Virulence In Vivo.,Romano M, van de Weerd R, Brouwer FC, Roviello GN, Lacroix R, Sparrius M, van den Brink-van Stempvoort G, Maaskant JJ, van der Sar AM, Appelmelk BJ, Geurtsen JJ, Berisio R Structure. 2014 May 6;22(5):719-30. doi: 10.1016/j.str.2014.01.014. Epub 2014 Apr, 3. PMID:24704253<ref>PMID:24704253</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Appelmelk, B J.]]
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[[Category: Large Structures]]
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[[Category: Berisio, R.]]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Brouwer, F C.C.]]
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[[Category: Appelmelk BJ]]
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[[Category: Geurtsen, J J.]]
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[[Category: Berisio R]]
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[[Category: Lacroix, R.]]
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[[Category: Brouwer FCC]]
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[[Category: Maaskant, J J.]]
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[[Category: Geurtsen JJ]]
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[[Category: Romano, M.]]
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[[Category: Lacroix R]]
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[[Category: Roviello, G N.]]
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[[Category: Maaskant JJ]]
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[[Category: Sar, A M.van der.]]
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[[Category: Romano M]]
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[[Category: Sparrius, M.]]
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[[Category: Roviello GN]]
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[[Category: Stempvoort, G van den Brink-van.]]
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[[Category: Sparrius M]]
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[[Category: Weerd, R van de.]]
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[[Category: Van de Weerd R]]
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[[Category: Alpha/beta fold]]
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[[Category: Van den Brink-van Stempvoort G]]
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[[Category: Exoribonuclease]]
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[[Category: Van der Sar AM]]
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[[Category: Hydrolase]]
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Current revision

Crystal structure of RNase AS from M tuberculosis in complex with UMP

PDB ID 4okk

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