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| | ==Structure of crystal form I of TP0453== | | ==Structure of crystal form I of TP0453== |
| - | <StructureSection load='3k8h' size='340' side='right' caption='[[3k8h]], [[Resolution|resolution]] 2.39Å' scene=''> | + | <StructureSection load='3k8h' size='340' side='right'caption='[[3k8h]], [[Resolution|resolution]] 2.39Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3k8h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K8H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3K8H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3k8h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Treponema_pallidum Treponema pallidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3K8H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3K8H FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene><br> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.39Å</td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3k8g|3k8g]], [[3k8i|3k8i]], [[3k8j|3k8j]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">30klp, TP_0453 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=160 Treponema pallidum])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3k8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k8h OCA], [https://pdbe.org/3k8h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3k8h RCSB], [https://www.ebi.ac.uk/pdbsum/3k8h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3k8h ProSAT]</span></td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3k8h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3k8h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3k8h RCSB], [http://www.ebi.ac.uk/pdbsum/3k8h PDBsum]</span></td></tr> | + | </table> |
| - | <table> | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/TP453_TREPA TP453_TREPA] Might be involved in ligand transport, alters membrane permeability at acidic pH (4.0 to 5.5) (PubMed:21965687). Incubation of the non-lipidated form with lipid vesicles increases their permeability (PubMed:16159783).<ref>PMID:16159783</ref> <ref>PMID:21965687</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | The Transition from Closed to Open Conformation of Treponema pallidum Outer Membrane-associated Lipoprotein TP0453 Involves Membrane Sensing and Integration by Two Amphipathic Helices.,Luthra A, Zhu G, Desrosiers DC, Eggers CH, Mulay V, Anand A, McArthur FA, Romano FB, Caimano MJ, Heuck AP, Malkowski MG, Radolf JD J Biol Chem. 2011 Dec 2;286(48):41656-68. Epub 2011 Sep 29. PMID:21965687<ref>PMID:21965687</ref> | | The Transition from Closed to Open Conformation of Treponema pallidum Outer Membrane-associated Lipoprotein TP0453 Involves Membrane Sensing and Integration by Two Amphipathic Helices.,Luthra A, Zhu G, Desrosiers DC, Eggers CH, Mulay V, Anand A, McArthur FA, Romano FB, Caimano MJ, Heuck AP, Malkowski MG, Radolf JD J Biol Chem. 2011 Dec 2;286(48):41656-68. Epub 2011 Sep 29. PMID:21965687<ref>PMID:21965687</ref> |
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 3k8h" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Large Structures]] |
| | [[Category: Treponema pallidum]] | | [[Category: Treponema pallidum]] |
| - | [[Category: Desrosiers, D.]] | + | [[Category: Desrosiers D]] |
| - | [[Category: Koszelak-Rosenblum, M.]] | + | [[Category: Koszelak-Rosenblum M]] |
| - | [[Category: Luthra, A.]] | + | [[Category: Luthra A]] |
| - | [[Category: Malkowski, M G.]] | + | [[Category: Malkowski MG]] |
| - | [[Category: Mulay, V.]] | + | [[Category: Mulay V]] |
| - | [[Category: Radolf, J D.]] | + | [[Category: Radolf JD]] |
| - | [[Category: Zhu, G.]] | + | [[Category: Zhu G]] |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Outer membrane protein]]
| + | |
| - | [[Category: Treponema pallidum]]
| + | |
| Structural highlights
Function
TP453_TREPA Might be involved in ligand transport, alters membrane permeability at acidic pH (4.0 to 5.5) (PubMed:21965687). Incubation of the non-lipidated form with lipid vesicles increases their permeability (PubMed:16159783).[1] [2]
Publication Abstract from PubMed
The molecular architecture and composition of the outer membrane (OM) of Treponema pallidum (Tp), the noncultivable agent of venereal syphilis, differ considerably from those of typical Gram-negative bacteria. Several years ago we described TP0453, the only lipoprotein associated with the inner leaflet of the Tp OM. Whereas polypeptides of other treponemal lipoproteins are hydrophilic, non-lipidated TP0453 can integrate into membranes, a property attributed to its multiple amphipathic helices (AHs). Furthermore, membrane integration of the TP0453 polypeptide was found to increase membrane permeability, suggesting the molecule functions in a porin-like manner. To better understand the mechanism of membrane integration of TP0453 and its physiological role in Tp OM biogenesis, we solved its crystal structure and used mutagenesis to identify membrane insertion elements. The crystal structure of TP0453 consists of an alpha/beta/alpha-fold and includes five stably folded AHs. In high concentrations of detergent, TP0453 transitions from a closed to open conformation by lateral movement of two groups of AHs, exposing a large hydrophobic cavity. Triton X-114 phase partitioning, liposome floatation assay, and bis-1-anilino-8-naphthalenesulfonate binding revealed that two adjacent AHs are critical for membrane sensing/integration. Using terbium-dipicolinic acid complex-loaded large unilamellar vesicles, we found that TP0453 increased efflux of fluorophore only at acidic pH. Gel filtration and cross-linking experiments demonstrated that one AH critical for membrane sensing/insertion also forms a dimeric interface. Based on structural dynamics and comparison with Mycobacterium tuberculosis lipoproteins LprG and LppX, we propose that TP0453 functions as a carrier of lipids, glycolipids, and/or derivatives during OM biogenesis.
The Transition from Closed to Open Conformation of Treponema pallidum Outer Membrane-associated Lipoprotein TP0453 Involves Membrane Sensing and Integration by Two Amphipathic Helices.,Luthra A, Zhu G, Desrosiers DC, Eggers CH, Mulay V, Anand A, McArthur FA, Romano FB, Caimano MJ, Heuck AP, Malkowski MG, Radolf JD J Biol Chem. 2011 Dec 2;286(48):41656-68. Epub 2011 Sep 29. PMID:21965687[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hazlett KR, Cox DL, Decaffmeyer M, Bennett MP, Desrosiers DC, La Vake CJ, La Vake ME, Bourell KW, Robinson EJ, Brasseur R, Radolf JD. TP0453, a concealed outer membrane protein of Treponema pallidum, enhances membrane permeability. J Bacteriol. 2005 Sep;187(18):6499-508. PMID:16159783 doi:10.1128/JB.187.18.6499-6508.2005
- ↑ Luthra A, Zhu G, Desrosiers DC, Eggers CH, Mulay V, Anand A, McArthur FA, Romano FB, Caimano MJ, Heuck AP, Malkowski MG, Radolf JD. The Transition from Closed to Open Conformation of Treponema pallidum Outer Membrane-associated Lipoprotein TP0453 Involves Membrane Sensing and Integration by Two Amphipathic Helices. J Biol Chem. 2011 Dec 2;286(48):41656-68. Epub 2011 Sep 29. PMID:21965687 doi:10.1074/jbc.M111.305284
- ↑ Luthra A, Zhu G, Desrosiers DC, Eggers CH, Mulay V, Anand A, McArthur FA, Romano FB, Caimano MJ, Heuck AP, Malkowski MG, Radolf JD. The Transition from Closed to Open Conformation of Treponema pallidum Outer Membrane-associated Lipoprotein TP0453 Involves Membrane Sensing and Integration by Two Amphipathic Helices. J Biol Chem. 2011 Dec 2;286(48):41656-68. Epub 2011 Sep 29. PMID:21965687 doi:10.1074/jbc.M111.305284
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