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3ia2
From Proteopedia
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==Pseudomonas fluorescens esterase complexed to the R-enantiomer of a sulfonate transition state analog== | ==Pseudomonas fluorescens esterase complexed to the R-enantiomer of a sulfonate transition state analog== | ||
| - | <StructureSection load='3ia2' size='340' side='right' caption='[[3ia2]], [[Resolution|resolution]] 1.65Å' scene=''> | + | <StructureSection load='3ia2' size='340' side='right'caption='[[3ia2]], [[Resolution|resolution]] 1.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3ia2]] is a 6 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3ia2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IA2 FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=J6Z:(2R)-BUTANE-2-SULFONATE'>J6Z</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=J6Z:(2R)-BUTANE-2-SULFONATE'>J6Z</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ia2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ia2 OCA], [https://pdbe.org/3ia2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ia2 RCSB], [https://www.ebi.ac.uk/pdbsum/3ia2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ia2 ProSAT]</span></td></tr> |
| - | <table> | + | </table> |
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/ESTE_PSEFL ESTE_PSEFL] Bifunctional enzyme, capable of both ester hydrolysis and halogenation. Has a low bromoperoxidase activity. Acts on many phenolic esters. | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/3ia2_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ia/3ia2_consurf.spt"</scriptWhenChecked> |
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ia2 ConSurf]. |
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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Different active-site loop orientation in serine hydrolases versus acyltransferases.,Jiang Y, Morley KL, Schrag JD, Kazlauskas RJ Chembiochem. 2011 Mar 21;12(5):768-76. doi: 10.1002/cbic.201000693. Epub, 2011 Feb 23. PMID:21351219<ref>PMID:21351219</ref> | Different active-site loop orientation in serine hydrolases versus acyltransferases.,Jiang Y, Morley KL, Schrag JD, Kazlauskas RJ Chembiochem. 2011 Mar 21;12(5):768-76. doi: 10.1002/cbic.201000693. Epub, 2011 Feb 23. PMID:21351219<ref>PMID:21351219</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
</div> | </div> | ||
| + | <div class="pdbe-citations 3ia2" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Pseudomonas fluorescens]] | [[Category: Pseudomonas fluorescens]] | ||
| - | [[Category: Jiang | + | [[Category: Jiang Y]] |
| - | [[Category: Kazlauskas | + | [[Category: Kazlauskas RJ]] |
| - | [[Category: Morley | + | [[Category: Morley K]] |
| - | [[Category: Schrag | + | [[Category: Schrag JD]] |
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Current revision
Pseudomonas fluorescens esterase complexed to the R-enantiomer of a sulfonate transition state analog
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