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| | ==The crystal structure of enterotoxigenic Escherichia coli GspC-GspD complex from the type II secretion system== | | ==The crystal structure of enterotoxigenic Escherichia coli GspC-GspD complex from the type II secretion system== |
| - | <StructureSection load='3oss' size='340' side='right' caption='[[3oss]], [[Resolution|resolution]] 2.63Å' scene=''> | + | <StructureSection load='3oss' size='340' side='right'caption='[[3oss]], [[Resolution|resolution]] 2.63Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3oss]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OSS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3oss]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_ETEC_H10407 Escherichia coli ETEC H10407]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OSS FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene><br> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.63Å</td></tr> |
| - | <tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GSPC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), GSPD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3oss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oss OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3oss RCSB], [http://www.ebi.ac.uk/pdbsum/3oss PDBsum]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3oss FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3oss OCA], [https://pdbe.org/3oss PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3oss RCSB], [https://www.ebi.ac.uk/pdbsum/3oss PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3oss ProSAT]</span></td></tr> |
| - | <table> | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/GSPD2_ECOH1 GSPD2_ECOH1] Part of a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of folded proteins across the outer membrane (Probable). This subunit forms the outer membrane channel (Probable).<ref>PMID:22585966</ref> <ref>PMID:23820381</ref> <ref>PMID:29632366</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | Structural and functional studies on the interaction of GspC and GspD in the type II secretion system.,Korotkov KV, Johnson TL, Jobling MG, Pruneda J, Pardon E, Heroux A, Turley S, Steyaert J, Holmes RK, Sandkvist M, Hol WG PLoS Pathog. 2011 Sep;7(9):e1002228. Epub 2011 Sep 8. PMID:21931548<ref>PMID:21931548</ref> | | Structural and functional studies on the interaction of GspC and GspD in the type II secretion system.,Korotkov KV, Johnson TL, Jobling MG, Pruneda J, Pardon E, Heroux A, Turley S, Steyaert J, Holmes RK, Sandkvist M, Hol WG PLoS Pathog. 2011 Sep;7(9):e1002228. Epub 2011 Sep 8. PMID:21931548<ref>PMID:21931548</ref> |
| | | | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
| | </div> | | </div> |
| | + | <div class="pdbe-citations 3oss" style="background-color:#fffaf0;"></div> |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Escherichia coli]] | + | [[Category: Escherichia coli ETEC H10407]] |
| - | [[Category: Hol, W G.J.]] | + | [[Category: Large Structures]] |
| - | [[Category: Korotkov, K V.]] | + | [[Category: Hol WGJ]] |
| - | [[Category: Pruneda, J.]] | + | [[Category: Korotkov KV]] |
| - | [[Category: General secretory pathway]] | + | [[Category: Pruneda J]] |
| - | [[Category: Hr domain]]
| + | |
| - | [[Category: Lanthanide-binding tag]]
| + | |
| - | [[Category: Protein transport]]
| + | |
| - | [[Category: Secretin]]
| + | |
| Structural highlights
Function
GSPD2_ECOH1 Part of a type II secretion system (T2SS, formerly general secretion pathway, GSP) for the export of folded proteins across the outer membrane (Probable). This subunit forms the outer membrane channel (Probable).[1] [2] [3]
Publication Abstract from PubMed
Type II secretion systems (T2SSs) are critical for secretion of many proteins from Gram-negative bacteria. In the T2SS, the outer membrane secretin GspD forms a multimeric pore for translocation of secreted proteins. GspD and the inner membrane protein GspC interact with each other via periplasmic domains. Three different crystal structures of the homology region domain of GspC (GspC(HR)) in complex with either two or three domains of the N-terminal region of GspD from enterotoxigenic Escherichia coli show that GspC(HR) adopts an all-beta topology. N-terminal beta-strands of GspC and the N0 domain of GspD are major components of the interface between these inner and outer membrane proteins from the T2SS. The biological relevance of the observed GspC-GspD interface is shown by analysis of variant proteins in two-hybrid studies and by the effect of mutations in homologous genes on extracellular secretion and subcellular distribution of GspC in Vibrio cholerae. Substitutions of interface residues of GspD have a dramatic effect on the focal distribution of GspC in V. cholerae. These studies indicate that the GspC(HR)-GspD(N0) interactions observed in the crystal structure are essential for T2SS function. Possible implications of our structures for the stoichiometry of the T2SS and exoprotein secretion are discussed.
Structural and functional studies on the interaction of GspC and GspD in the type II secretion system.,Korotkov KV, Johnson TL, Jobling MG, Pruneda J, Pardon E, Heroux A, Turley S, Steyaert J, Holmes RK, Sandkvist M, Hol WG PLoS Pathog. 2011 Sep;7(9):e1002228. Epub 2011 Sep 8. PMID:21931548[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Strozen TG, Li G, Howard SP. YghG (GspSbeta) is a novel pilot protein required for localization of the GspSbeta type II secretion system secretin of enterotoxigenic Escherichia coli. Infect Immun. 2012 Aug;80(8):2608-22. doi: 10.1128/IAI.06394-11. Epub 2012 May , 14. PMID:22585966 doi:http://dx.doi.org/10.1128/IAI.06394-11
- ↑ Korotkov KV, Delarosa JR, Hol WG. A dodecameric ring-like structure of the N0 domain of the type II secretin from enterotoxigenic Escherichia coli. J Struct Biol. 2013 Jun 29. pii: S1047-8477(13)00168-8. doi:, 10.1016/j.jsb.2013.06.013. PMID:23820381 doi:10.1016/j.jsb.2013.06.013
- ↑ Yin M, Yan Z, Li X. Structural insight into the assembly of the type II secretion system pilotin-secretin complex from enterotoxigenic Escherichia coli. Nat Microbiol. 2018 May;3(5):581-587. doi: 10.1038/s41564-018-0148-0. Epub 2018, Apr 9. PMID:29632366 doi:http://dx.doi.org/10.1038/s41564-018-0148-0
- ↑ Korotkov KV, Johnson TL, Jobling MG, Pruneda J, Pardon E, Heroux A, Turley S, Steyaert J, Holmes RK, Sandkvist M, Hol WG. Structural and functional studies on the interaction of GspC and GspD in the type II secretion system. PLoS Pathog. 2011 Sep;7(9):e1002228. Epub 2011 Sep 8. PMID:21931548 doi:10.1371/journal.ppat.1002228
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