3n29
From Proteopedia
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==Crystal structure of carboxynorspermidine decarboxylase complexed with Norspermidine from Campylobacter jejuni== | ==Crystal structure of carboxynorspermidine decarboxylase complexed with Norspermidine from Campylobacter jejuni== | ||
| - | <StructureSection load='3n29' size='340' side='right' caption='[[3n29]], [[Resolution|resolution]] 1.90Å' scene=''> | + | <StructureSection load='3n29' size='340' side='right'caption='[[3n29]], [[Resolution|resolution]] 1.90Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3n29]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3n29]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni_81116 Campylobacter jejuni subsp. jejuni 81116]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3N29 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3N29 FirstGlance]. <br> |
| - | </td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NSD:N-(3-AMINOPROPYL)PROPANE-1,3-DIAMINE'>NSD</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NSD:N-(3-AMINOPROPYL)PROPANE-1,3-DIAMINE'>NSD</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | |
| - | <tr | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3n29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3n29 OCA], [https://pdbe.org/3n29 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3n29 RCSB], [https://www.ebi.ac.uk/pdbsum/3n29 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3n29 ProSAT]</span></td></tr> |
| - | + | </table> | |
| - | <table> | + | == Function == |
| + | [https://www.uniprot.org/uniprot/NSPC_CAMJ8 NSPC_CAMJ8] Catalyzes the decarboxylation of carboxynorspermidine and carboxyspermidine in vitro. In vivo, responsible for synthesizing spermidine, but not sym-norspermidine.<ref>PMID:20534592</ref> <ref>PMID:22025614</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
| - | <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/3n29_consurf.spt"</scriptWhenChecked> | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/n2/3n29_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/ | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
</jmolCheckbox> | </jmolCheckbox> | ||
| - | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/ | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3n29 ConSurf]. |
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
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Evolution of substrate specificity within a diverse family of beta/alpha-barrel-fold basic amino acid decarboxylases: X-ray structure determination of enzymes with specificity for L-arginine and carboxynorspermidine.,Deng X, Lee J, Michael AJ, Tomchick DR, Goldsmith EJ, Phillips MA J Biol Chem. 2010 Aug 13;285(33):25708-19. Epub 2010 Jun 8. PMID:20534592<ref>PMID:20534592</ref> | Evolution of substrate specificity within a diverse family of beta/alpha-barrel-fold basic amino acid decarboxylases: X-ray structure determination of enzymes with specificity for L-arginine and carboxynorspermidine.,Deng X, Lee J, Michael AJ, Tomchick DR, Goldsmith EJ, Phillips MA J Biol Chem. 2010 Aug 13;285(33):25708-19. Epub 2010 Jun 8. PMID:20534592<ref>PMID:20534592</ref> | ||
| - | From | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> |
</div> | </div> | ||
| + | <div class="pdbe-citations 3n29" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Campylobacter jejuni subsp. jejuni 81116]] | [[Category: Campylobacter jejuni subsp. jejuni 81116]] | ||
| - | [[Category: Deng | + | [[Category: Large Structures]] |
| - | [[Category: Goldsmith | + | [[Category: Deng X]] |
| - | [[Category: Lee | + | [[Category: Goldsmith EJ]] |
| - | [[Category: Michael | + | [[Category: Lee J]] |
| - | [[Category: Phillips | + | [[Category: Michael AJ]] |
| - | [[Category: Tomchick | + | [[Category: Phillips MA]] |
| - | + | [[Category: Tomchick DR]] | |
Current revision
Crystal structure of carboxynorspermidine decarboxylase complexed with Norspermidine from Campylobacter jejuni
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