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3nyq

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==Malonyl-CoA Ligase Ternary Product Complex with Methylmalonyl-CoA and AMP bound==
==Malonyl-CoA Ligase Ternary Product Complex with Methylmalonyl-CoA and AMP bound==
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<StructureSection load='3nyq' size='340' side='right' caption='[[3nyq]], [[Resolution|resolution]] 1.43&Aring;' scene=''>
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<StructureSection load='3nyq' size='340' side='right'caption='[[3nyq]], [[Resolution|resolution]] 1.43&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3nyq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3NYQ FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3nyq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3NYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3NYQ FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MCA:METHYLMALONYL-COENZYME+A'>MCA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.43&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ite|3ite]], [[3e7w|3e7w]], [[1t5d|1t5d]], [[2d1r|2d1r]], [[1ry2|1ry2]], [[1pg3|1pg3]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MCA:METHYLMALONYL-COENZYME+A'>MCA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MatB, SCC24.15, SCO2444 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3nyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nyq OCA], [https://pdbe.org/3nyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3nyq RCSB], [https://www.ebi.ac.uk/pdbsum/3nyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3nyq ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3nyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3nyq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3nyq RCSB], [http://www.ebi.ac.uk/pdbsum/3nyq PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/Q9L0A2_STRCO Q9L0A2_STRCO]
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== Publication Abstract from PubMed ==
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In vitro experiments with modular polyketide synthases (PKSs) are often limited by the availability of polyketide extender units. To determine the polyketide extender units that can be biocatalytically accessed via promiscuous malonyl-CoA ligases, structural and functional studies were conducted on Streptomyces coelicolor MatB. We demonstrate that this adenylate-forming enzyme is capable of producing most CoA-linked polyketide extender units as well as pantetheine- and N-acetylcysteamine-linked analogs useful for in vitro PKS studies. Two ternary product complex structures, one containing malonyl-CoA and AMP and the other containing (2R)-methylmalonyl-CoA and AMP, were solved to 1.45 A and 1.43 A resolution, respectively. MatB crystallized in the thioester-forming conformation, making extensive interactions with the bound extender unit products. This first structural characterization of an adenylate-forming enzyme that activates diacids reveals the molecular details for how malonate and its derivatives are accepted. The orientation of the alpha-methyl group of bound (2R)-methylmalonyl-CoA, indicates that it is necessary to epimerize alpha-substituted extender units formed by MatB before they can be accepted by PKS acyltransferase domains. We demonstrate the in vitro incorporation of methylmalonyl groups ligated by MatB to CoA, pantetheine, or N-acetylcysteamine into a triketide pyrone by the terminal module of the 6-deoxyerythronolide B synthase. Additionally, a means for quantitatively monitoring certain in vitro PKS reactions using MatB is presented.
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Enzymatic extender unit generation for in vitro polyketide synthase reactions: structural and functional showcasing of Streptomyces coelicolor MatB.,Hughes AJ, Keatinge-Clay A Chem Biol. 2011 Feb 25;18(2):165-76. PMID:21338915<ref>PMID:21338915</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces coelicolor]]
[[Category: Streptomyces coelicolor]]
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[[Category: Hughes, A J.]]
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[[Category: Hughes AJ]]
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[[Category: Keatinge-Clay, A T.]]
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[[Category: Keatinge-Clay AT]]
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[[Category: A/b topology ababa sandwich beta-barrel adenylate-forming enzyme fold]]
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[[Category: Ligase]]
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Current revision

Malonyl-CoA Ligase Ternary Product Complex with Methylmalonyl-CoA and AMP bound

PDB ID 3nyq

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