1am2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:31, 7 February 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1am2.gif|left|200px]]
 
-
{{Structure
+
==GYRA INTEIN FROM MYCOBACTERIUM XENOPI==
-
|PDB= 1am2 |SIZE=350|CAPTION= <scene name='initialview01'>1am2</scene>, resolution 2.2&Aring;
+
<StructureSection load='1am2' size='340' side='right'caption='[[1am2]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND=
+
<table><tr><td colspan='2'>[[1am2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_xenopi Mycobacterium xenopi]. The November 2010 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Inteins'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2010_11 10.2210/rcsb_pdb/mom_2010_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AM2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AM2 FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
-
|GENE= RTP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1789 Mycobacterium xenopi])
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1am2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1am2 OCA], [https://pdbe.org/1am2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1am2 RCSB], [https://www.ebi.ac.uk/pdbsum/1am2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1am2 ProSAT]</span></td></tr>
-
}}
+
</table>
-
 
+
== Function ==
-
'''GYRA INTEIN FROM MYCOBACTERIUM XENOPI'''
+
[https://www.uniprot.org/uniprot/GYRA_MYCXE GYRA_MYCXE] DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings (By similarity).
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
-
Several genes from prokaryotes and lower eukaryotes have been found to contain an in-frame open reading frame, which encodes for an internal protein (intein). Post-translationally, the internal polypeptide auto-splices and ligates the external sequences to yield a functional external protein (extein) and an intein. Most, but not all inteins, contain, apart from a splicing domain, a separate endonucleolytic domain that enables them to maintain their presence by a homing mechanism. We report here the crystal structure of an intein found in the gyrase A subunit from Mycobacterium xenopi at 2.2 A resolution. The structure contains an unusual beta-fold with the catalytic splice junctions at the ends of two adjacent antiparallel beta-strands. The arrangement of the active site residues Ser 1, Thr 72, His 75, His 197, and Asn 198 is consistent with a four-step mechanism for the cleavage-ligation reaction. Using site-directed mutagenesis, the N-terminal cysteine, proposed as the nucleophile in the first step of the splicing reaction, was changed to a Ser 1 and Ala 0, thus capturing the intein in a pre-spliced state.
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/am/1am2_consurf.spt"</scriptWhenChecked>
-
==About this Structure==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
1AM2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_xenopi Mycobacterium xenopi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AM2 OCA].
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==Reference==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1am2 ConSurf].
-
Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing., Klabunde T, Sharma S, Telenti A, Jacobs WR Jr, Sacchettini JC, Nat Struct Biol. 1998 Jan;5(1):31-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9437427 9437427]
+
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
 +
[[Category: Inteins]]
 +
[[Category: Large Structures]]
[[Category: Mycobacterium xenopi]]
[[Category: Mycobacterium xenopi]]
-
[[Category: Single protein]]
+
[[Category: RCSB PDB Molecule of the Month]]
-
[[Category: Klabunde, T.]]
+
[[Category: Klabunde T]]
-
[[Category: Sacchettini, J C.]]
+
[[Category: Sacchettini JC]]
-
[[Category: Sharma, S.]]
+
[[Category: Sharma S]]
-
[[Category: intein]]
+
-
[[Category: protein splicing]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 09:59:24 2008''
+

Current revision

GYRA INTEIN FROM MYCOBACTERIUM XENOPI

PDB ID 1am2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1am2"
Personal tools