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3qq6

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==The N-terminal DNA binding domain of SinR from Bacillus subtilis==
==The N-terminal DNA binding domain of SinR from Bacillus subtilis==
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<StructureSection load='3qq6' size='340' side='right' caption='[[3qq6]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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<StructureSection load='3qq6' size='340' side='right'caption='[[3qq6]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3qq6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QQ6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QQ6 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3qq6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QQ6 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1b0n|1b0n]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BSU24610, flaD, sin, sinR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qq6 OCA], [https://pdbe.org/3qq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qq6 RCSB], [https://www.ebi.ac.uk/pdbsum/3qq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qq6 ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qq6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qq6 RCSB], [http://www.ebi.ac.uk/pdbsum/3qq6 PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/SINR_BACSU SINR_BACSU] Negative as well as positive regulator of alternate developmental processes that are induced at the end of vegetative growth in response to nutrient depletion. Binds to the alkaline protease (aprE) gene at two sites. Also acts as a repressor of the key sporulation gene spo0A. Negatively regulates transcription of the eps operon, which is responsible for the biosynthesis of an exopolysaccharide involved in biofilm formation; therefore it could govern the transition between a state in which bacteria swim or swarm and a state in which bacteria assemble into multicellular communities. Acts with Hpr as a corepressor of epr expression. Also negatively regulates transcription of the lutABC operon, which is required for lactate utilization. Repressor activity is regulated by SinI.<ref>PMID:1898931</ref> <ref>PMID:7642487</ref> <ref>PMID:15661000</ref> <ref>PMID:16923912</ref>
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== Publication Abstract from PubMed ==
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sinR encodes a tetrameric repressor of genes required for biofilm formation in Bacillus subtilis. sinI, which is transcribed under Spo0A control, encodes a dimeric protein that binds to SinR to form a SinR-SinI heterodimer in which the DNA-binding functions of SinR are abrogated and repression of biofilm genes is relieved. The heterodimer-forming surface comprises residues conserved between SinR and SinI. Each forms a pair of alpha-helices that hook together to form an intermolecular four-helix bundle. Here, we are interested in the assembly of the SinR tetramer and its binding to DNA. Size-exclusion chromatography with multi-angle laser light scattering and crystallographic analysis reveal that a DNA-binding fragment of SinR (residues 1-69) is a monomer, while a SinI-binding fragment (residues 74-111) is a tetramer arranged as a dimer of dimers. The SinR(74-111) chain forms two alpha-helices with the organisation of the dimer similar to that observed in the SinR-SinI complex. The tetramer is formed through interactions of residues at the C-termini of the four chains. A model of the intact SinR tetramer in which the DNA binding domains surround the tetramerisation core was built. Fluorescence anisotropy and surface plasmon resonance experiments showed that SinR binds to an oligonucleotide duplex, 5'-TTTGTTCTCTAAAGAGAACTTA-3', containing a pair of SinR consensus sequences in inverted orientation with a K(d) of 300 nM. The implications of these data for promoter binding and the curious quaternary structural transitions of SinR upon binding to (i) SinI and (ii) the SinR-like protein SlrR, which "repurposes" SinR as a repressor of autolysin and motility genes, are discussed.
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Structure and Organisation of SinR, the Master Regulator of Biofilm Formation in Bacillus subtilis.,Colledge VL, Fogg MJ, Levdikov VM, Leech A, Dodson EJ, Wilkinson AJ J Mol Biol. 2011 Aug 19;411(3):597-613. Epub 2011 Jun 25. PMID:21708175<ref>PMID:21708175</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Colledge, V.]]
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[[Category: Large Structures]]
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[[Category: Dodson, E J.]]
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[[Category: Colledge V]]
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[[Category: Fogg, M J.]]
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[[Category: Dodson EJ]]
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[[Category: Levdikov, V M.]]
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[[Category: Fogg MJ]]
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[[Category: Wilkinson, A J.]]
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[[Category: Levdikov VM]]
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[[Category: Biofilm]]
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[[Category: Wilkinson AJ]]
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[[Category: Helix-turn-helix motif]]
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[[Category: Repressor]]
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[[Category: Sini]]
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[[Category: Transcription]]
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[[Category: Transcriptional regulator]]
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Current revision

The N-terminal DNA binding domain of SinR from Bacillus subtilis

PDB ID 3qq6

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