3qh2

<StructureSection load='3qh2' size='340' side='right' caption='[[3qh2]], [[Resolution|resolution]] 2.23&Aring;' scene=''>

<table><tr><td colspan='2'>[[3qh2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QH2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QH2 FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3NM:4-METHYL-5-[2-(PHOSPHONOOXY)ETHYL]-1,3-THIAZOLE-2-CARBOXYLIC+ACID'>3NM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>

<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tenI, BSU11660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])</td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qh2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qh2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qh2 RCSB], [http://www.ebi.ac.uk/pdbsum/3qh2 PDBsum]</span></td></tr>

<table>

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== Publication Abstract from PubMed ==

In many bacteria tenI is found clustered with genes involved in thiamin thiazole biosynthesis. However, while TenI shows high sequence similarity with thiamin phosphate synthase, the purified protein has no thiamin phosphate synthase activity and the role of this enzyme in thiamin biosynthesis remains unknown. In this paper, we identify the function of TenI as a thiazole tautomerase, describe the structure of the enzyme complexed with its reaction product, identify the substrate phosphate and histidine 122 as the acid/base residues involved in catalysis and propose a mechanism for the reaction. The identification of the function of TenI completes the identification of all of the enzymes needed for thiamin biosynthesis by the major bacterial pathway.

 

A Missing Enzyme in Thiamin Thiazole Biosynthesis: Identification of TenI as a Thiazole Tautomerase.,Hazra AB, Han Y, Chatterjee A, Zhang Y, Lai RY, Ealick SE, Begley TP J Am Chem Soc. 2011 May 2. PMID:21534620<ref>PMID:21534620</ref>

 

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: Begley, T P.]]

[[Category: Chatterjee, A.]]

[[Category: Ealick, S E.]]

[[Category: Han, Y.]]

[[Category: Hazra, A.]]

[[Category: Lai, R.]]

[[Category: Zhang, Y.]]

[[Category: Isomerase]]