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3qdt

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==Structure of Boletus edulis lectin in complex with T-antigen disaccharide==
==Structure of Boletus edulis lectin in complex with T-antigen disaccharide==
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<StructureSection load='3qdt' size='340' side='right' caption='[[3qdt]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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<StructureSection load='3qdt' size='340' side='right'caption='[[3qdt]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3qdt]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Boletus_edulis Boletus edulis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QDT FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3qdt]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Boletus_edulis Boletus edulis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QDT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QDT FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=PRD_900084:Thomsen-Friedenreich+antigen'>PRD_900084</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qds|3qds]], [[3qdv|3qdv]], [[3qdw|3qdw]], [[3qdx|3qdx]], [[3qdy|3qdy]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdt OCA], [https://pdbe.org/3qdt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qdt RCSB], [https://www.ebi.ac.uk/pdbsum/3qdt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qdt ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qdt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qdt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qdt RCSB], [http://www.ebi.ac.uk/pdbsum/3qdt PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/BEL_BOLED BEL_BOLED]
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== Publication Abstract from PubMed ==
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A novel lectin has been isolated from the fruiting bodies of the common edible mushroom Boletus edulis (king bolete, penny bun, porcino, or cep) by affinity chromatography on a chitin column. We propose for the lectin the name BEL (Boletus edulis lectin). BEL inhibits selectively the proliferation of several malignant cell lines and binds the neoplastic cell specific T-antigen disaccharide, Galbeta1-3GalNAc. The lectin was structurally characterized: the molecule is a homotetramer and the 142 amino acid sequence of the chains was determined. The protein belongs to the saline-soluble family of mushroom fruiting body specific lectins. BEL was also crystallized and its three-dimensional structure was determined by X-ray diffraction to 1.15 A resolution. The structure is similar to that of Agaricus bisporus lectin. Using the appropriate co-crystals, the interactions of BEL with specific mono and disaccharides were also studied by X-ray diffraction. The six structures of carbohydrate complexes reported here provide details of the interactions of the ligands with the lectin and shed light on the selectivity of the two distinct binding sites present in each protomer.
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STRUCTURE OF A LECTIN WITH ANTITUMORAL PROPERTIES IN KING BOLETE (Boletus edulis) MUSHROOMS.,Bovi M, Carrizo ME, Capaldi S, Perduca M, Chiarelli LR, Galliano M, Monaco HL Glycobiology. 2011 Feb 8. PMID:21303815<ref>PMID:21303815</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Boletus edulis]]
[[Category: Boletus edulis]]
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[[Category: Bovi, M.]]
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[[Category: Large Structures]]
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[[Category: Capaldi, S.]]
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[[Category: Bovi M]]
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[[Category: Carrizo, M E.]]
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[[Category: Capaldi S]]
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[[Category: Chiarelli, L R.]]
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[[Category: Carrizo ME]]
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[[Category: Galliano, M.]]
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[[Category: Chiarelli LR]]
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[[Category: Monaco, H L.]]
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[[Category: Galliano M]]
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[[Category: Perduca, M.]]
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[[Category: Monaco HL]]
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[[Category: Boletus eduli]]
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[[Category: Perduca M]]
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[[Category: Carbohydrate]]
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[[Category: Lectin]]
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[[Category: Mushroom]]
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[[Category: Sugar binding protein]]
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[[Category: T-antigen]]
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Current revision

Structure of Boletus edulis lectin in complex with T-antigen disaccharide

PDB ID 3qdt

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