3r5z

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==Structure of a Deazaflavin-dependent reductase from Nocardia farcinica, with co-factor F420==
==Structure of a Deazaflavin-dependent reductase from Nocardia farcinica, with co-factor F420==
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<StructureSection load='3r5z' size='340' side='right' caption='[[3r5z]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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<StructureSection load='3r5z' size='340' side='right'caption='[[3r5z]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3r5z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nocardia_farcinica Nocardia farcinica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R5Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R5Z FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3r5z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nocardia_farcinica Nocardia farcinica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R5Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R5Z FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F42:COENZYME+F420'>F42</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.503&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3r5l|3r5l]], [[3r5p|3r5p]], [[3r5r|3r5r]], [[3r5w|3r5w]], [[3r5y|3r5y]], [[3h96|3h96]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F42:COENZYME+F420'>F42</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NFA_18080 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37329 Nocardia farcinica])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r5z OCA], [https://pdbe.org/3r5z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r5z RCSB], [https://www.ebi.ac.uk/pdbsum/3r5z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r5z ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r5z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r5z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r5z RCSB], [http://www.ebi.ac.uk/pdbsum/3r5z PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/Q5YYT7_NOCFA Q5YYT7_NOCFA]
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== Publication Abstract from PubMed ==
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Tuberculosis continues to be a global health threat, making bicyclic nitroimidazoles an important new class of therapeutics. A deazaflavin-dependent nitroreductase (Ddn) from Mycobacterium tuberculosis catalyzes the reduction of nitroimidazoles such as PA-824, resulting in intracellular release of lethal reactive nitrogen species. The N-terminal 30 residues of Ddn are functionally important but are flexible or access multiple conformations, preventing structural characterization of the full-length, enzymatically active enzyme. Several structures were determined of a truncated, inactive Ddn protein core with and without bound F(420) deazaflavin coenzyme as well as of a catalytically competent homolog from Nocardia farcinica. Mutagenesis studies based on these structures identified residues important for binding of F(420) and PA-824. The proposed orientation of the tail of PA-824 toward the N terminus of Ddn is consistent with current structure-activity relationship data.
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Structure of Ddn, the deazaflavin-dependent nitroreductase from Mycobacterium tuberculosis involved in bioreductive activation of PA-824.,Cellitti SE, Shaffer J, Jones DH, Mukherjee T, Gurumurthy M, Bursulaya B, Boshoff HI, Choi I, Nayyar A, Lee YS, Cherian J, Niyomrattanakit P, Dick T, Manjunatha UH, Barry CE 3rd, Spraggon G, Geierstanger BH Structure. 2012 Jan 11;20(1):101-12. PMID:22244759<ref>PMID:22244759</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Large Structures]]
[[Category: Nocardia farcinica]]
[[Category: Nocardia farcinica]]
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[[Category: Barry, C E.]]
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[[Category: Barry CE]]
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[[Category: Boshoff, H I.M.]]
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[[Category: Boshoff HIM]]
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[[Category: Bursulaya, B.]]
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[[Category: Bursulaya B]]
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[[Category: Cellitti, S E.]]
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[[Category: Cellitti SE]]
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[[Category: Cherian, J.]]
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[[Category: Cherian J]]
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[[Category: Choi, I.]]
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[[Category: Choi I]]
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[[Category: Dick, T.]]
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[[Category: Dick T]]
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[[Category: Geierstanger, B H.]]
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[[Category: Geierstanger BH]]
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[[Category: Gurumurthy, M.]]
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[[Category: Gurumurthy M]]
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[[Category: Jones, D H.]]
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[[Category: Jones DH]]
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[[Category: Lee, Y S.]]
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[[Category: Lee YS]]
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[[Category: Manjunatha, U H.]]
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[[Category: Manjunatha UH]]
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[[Category: Mukherjee, T.]]
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[[Category: Mukherjee T]]
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[[Category: Nayya, A.]]
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[[Category: Nayya A]]
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[[Category: Niyomrattanakit, P.]]
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[[Category: Niyomrattanakit P]]
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[[Category: Shaffer, J.]]
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[[Category: Shaffer J]]
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[[Category: Spraggon, G.]]
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[[Category: Spraggon G]]
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[[Category: Deazaflavin-dependent reductase]]
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[[Category: Duf385]]
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[[Category: F420]]
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[[Category: F420-dependent reductase]]
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[[Category: Fdr]]
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[[Category: Split barrel-like fold]]
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[[Category: Unknown function]]
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Current revision

Structure of a Deazaflavin-dependent reductase from Nocardia farcinica, with co-factor F420

PDB ID 3r5z

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