3szx

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:58, 1 March 2024) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal Structure of the Triplet Repeat in Myotonic Dystrophy Reveals Heterogeneous 1x1 Nucleotide UU Internal Loop Conformations==
==Crystal Structure of the Triplet Repeat in Myotonic Dystrophy Reveals Heterogeneous 1x1 Nucleotide UU Internal Loop Conformations==
-
<StructureSection load='3szx' size='340' side='right' caption='[[3szx]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+
<StructureSection load='3szx' size='340' side='right'caption='[[3szx]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3szx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SZX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SZX FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3szx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SZX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SZX FirstGlance]. <br>
-
</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3syw|3syw]]</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.204&#8491;</td></tr>
-
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3szx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3szx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3szx RCSB], [http://www.ebi.ac.uk/pdbsum/3szx PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3szx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3szx OCA], [https://pdbe.org/3szx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3szx RCSB], [https://www.ebi.ac.uk/pdbsum/3szx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3szx ProSAT]</span></td></tr>
-
<table>
+
</table>
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
RNA internal loops often display a variety of conformations in solution. Herein, we visualize conformational heterogeneity in the context of the 5'CUG/3'GUC repeat motif present in the RNA that causes myotonic dystrophy type 1 (DM1). Specifically, two crystal structures of a model DM1 triplet repeating construct, 5'r[UUGGGC(CUG)(3)GUCC](2), refined to 2.20 and 1.52 A resolution are disclosed. Here, differences in the orientation of the 5' dangling UU end between the two structures induce changes in the backbone groove width, which reveals that noncanonical 1 x 1 nucleotide UU internal loops can display an ensemble of pairing conformations. In the 2.20 A structure, CUGa, the 5' UU forms a one hydrogen-bonded pair with a 5' UU of a neighboring helix in the unit cell to form a pseudoinfinite helix. The central 1 x 1 nucleotide UU internal loop has no hydrogen bonds, while the terminal 1 x 1 nucleotide UU internal loops each form a one-hydrogen bond pair. In the 1.52 A structure, CUGb, the 5' UU dangling end is tucked into the major groove of the duplex. While the canonically paired bases show no change in base pairing, in CUGb the terminal 1 x 1 nucleotide UU internal loops now form two hydrogen-bonded pairs. Thus, the shift in the major groove induced by the 5' UU dangling end alters noncanonical base patterns. Collectively, these structures indicate that 1 x 1 nucleotide UU internal loops in DM1 may sample multiple conformations in vivo. This observation has implications for the recognition of this RNA, and other repeating transcripts, by protein and small molecule ligands.
+
-
 
+
-
Myotonic dystrophy type 1 RNA crystal structures reveal heterogeneous 1 x 1 nucleotide UU internal loop conformations.,Kumar A, Park H, Fang P, Parkesh R, Guo M, Nettles KW, Disney MD Biochemistry. 2011 Nov 15;50(45):9928-35. Epub 2011 Oct 20. PMID:21988728<ref>PMID:21988728</ref>
+
-
 
+
-
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Disney, M D.]]
+
[[Category: Large Structures]]
-
[[Category: Guo, M.]]
+
[[Category: Disney MD]]
-
[[Category: Kumar, A.]]
+
[[Category: Guo M]]
-
[[Category: Nettles, K W.]]
+
[[Category: Kumar A]]
-
[[Category: Park, H.]]
+
[[Category: Nettles KW]]
-
[[Category: Parkesh, R.]]
+
[[Category: Park H]]
-
[[Category: Pengfei, F.]]
+
[[Category: Parkesh R]]
-
[[Category: Cug repeat rna]]
+
[[Category: Pengfei F]]
-
[[Category: Rna]]
+

Current revision

Crystal Structure of the Triplet Repeat in Myotonic Dystrophy Reveals Heterogeneous 1x1 Nucleotide UU Internal Loop Conformations

PDB ID 3szx

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3szx"
Personal tools